Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,790,264	T→C	E327E (GAA→GAG)	ydiU ←	UPF0061 family protein YdiU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,790,264	0	T	C	100.0%	88.9 / NA	26	E327E (GAA→GAG)	ydiU	UPF0061 family protein YdiU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/11);  total (15/11)

CAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGC  >  NC_000913/1790189‑1790339                                                                            |                                                                            cAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCtctc                                                                         >  2:288493/1‑80 (MQ=255)       gAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATg                                                                  >  1:288500/1‑81 (MQ=255)            attcattcAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTca                                                             <  2:288473/81‑1 (MQ=255)              tcattcaGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTcaa                                                            >  2:288498/1‑80 (MQ=255)                attcaGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTcaacaa                                                         <  2:288474/81‑1 (MQ=255)                     gTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACct                                                    >  2:288502/1‑81 (MQ=255)                      tAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACctg                                                   <  2:288475/81‑1 (MQ=255)                       aGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACctgc                                                  >  1:288496/1‑81 (MQ=255)                        gcgcGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACctgct                                                 >  2:288510/1‑81 (MQ=255)                        gcgcGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACctgct                                                 <  2:288476/81‑1 (MQ=255)                         cgcgTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAGCctgctg                                                <  2:288477/81‑1 (MQ=255)                                tCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCt                                          <  2:288479/80‑1 (MQ=255)                                tCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCt                                          <  2:288478/80‑1 (MQ=255)                                    ctTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTc                                       >  1:288495/1‑79 (MQ=255)                                       tttGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAggg                                  >  1:288501/1‑81 (MQ=255)                                        ttGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAggg                                  <  2:288480/80‑1 (MQ=255)                                          gCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGcc                                <  2:288481/80‑1 (MQ=255)                                               gTCATGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTc                          <  2:288482/81‑1 (MQ=255)                                                  aTGAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAgg                       >  1:288511/1‑81 (MQ=255)                                                    gAAGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAggg                      >  2:288516/1‑80 (MQ=255)                                                     aaGCCCAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGc                     <  1:288483/80‑1 (MQ=255)                                                        cccAGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATc                  >  1:288505/1‑80 (MQ=255)                                                           aGTTTCTGCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACg              >  1:288508/1‑81 (MQ=255)                                                                  gCCGCATCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGcaaca        >  1:288506/1‑80 (MQ=255)                                                                       aTCCGCTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGc  >  1:288509/1‑81 (MQ=255)                                                                        tCCGCTCTCCATAATGCATCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGc  >  1:288504/1‑80 (MQ=255)                                                                            |                                                                            CAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGC  >  NC_000913/1790189‑1790339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑