Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,796,866	T→C	Y360C (TAT→TGT)	pheT ←	phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,796,866	0	T	C	100.0%	95.1 / NA	28	Y360C (TAT→TGT)	pheT	phenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/12);  total (16/12)

CCACCGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACG  >  NC_000913/1796788‑1796938                                                                               |                                                                         ccaccGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACaa                                                                        >  1:289298/1‑81 (MQ=255) ccaccGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACaa                                                                        >  1:289300/1‑81 (MQ=255)               gATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATc                                                          >  2:289303/1‑81 (MQ=255)               gATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATc                                                          >  1:289301/1‑81 (MQ=255)                aTCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATTAACGCCACGCTCACAACGGTGAGACGCATCg                                                         >  2:289315/1‑81 (MQ=255)                   agcagACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGt                                                       <  1:289262/80‑1 (MQ=255)                     cagACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATg                                                    <  1:289263/81‑1 (MQ=255)                      agACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATg                                                    >  2:289313/1‑80 (MQ=255)                      agACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGc                                                   <  1:289264/81‑1 (MQ=255)                        aCGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAg                                                 <  1:289265/81‑1 (MQ=255)                        aCGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAg                                                 <  1:289266/81‑1 (MQ=255)                               gCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATg                                           <  1:289267/80‑1 (MQ=255)                                cACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATgac                                         >  2:289304/1‑81 (MQ=255)                                       cATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAg                                   >  1:289314/1‑80 (MQ=255)                                            cTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGAc                             >  1:289308/1‑81 (MQ=255)                                             tttGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGAcc                            >  1:289316/1‑81 (MQ=255)                                                   ctgcAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGAt                      <  2:289268/81‑1 (MQ=255)                                                     gcAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGa                       >  2:289305/1‑78 (MQ=255)                                                      cAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGa                   >  1:289306/1‑81 (MQ=255)                                                         tGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAg                <  2:289270/81‑1 (MQ=255)                                                         tGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAg                <  1:289269/81‑1 (MQ=255)                                                           ccGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCg              <  1:289271/81‑1 (MQ=255)                                                            cGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCgg             >  2:289321/1‑81 (MQ=255)                                                             ggATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCggg            >  2:289309/1‑81 (MQ=255)                                                               aTCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCt          <  1:289272/81‑1 (MQ=255)                                                                  aaCGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTa         >  1:289311/1‑79 (MQ=255)                                                                      ccACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAAc   <  1:289273/81‑1 (MQ=255)                                                                       cACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAAcg  >  2:289307/1‑81 (MQ=255)                                                                               |                                                                         CCACCGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACG  >  NC_000913/1796788‑1796938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑