Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,808,200	G→A	Q135Q (CAG→CAA)	yniA →	putative kinase YniA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,808,200	0	G	A	100.0%	72.1 / NA	22	Q135Q (CAG→CAA)	yniA	putative kinase YniA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/12);  total (10/12)

CGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTG  >  NC_000913/1808127‑1808273                                                                          |                                                                          cgcgTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAACCCAaca                                                                    >  1:290665/1‑81 (MQ=255)    gTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAACCCAACACCt                                                                 >  1:290673/1‑81 (MQ=255)         cATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCaa                                                            >  1:290667/1‑81 (MQ=255)             aaTGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGt                                                         <  2:290638/80‑1 (MQ=255)                 gAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTg                                                    <  2:290639/81‑1 (MQ=255)                   gCGACCAGCCACAATTTGGCCCTGATTTCGATAACCCGCTCTTCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGt                                                  <  1:290640/81‑1 (MQ=255)                    cGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTc                                                 <  1:290641/81‑1 (MQ=255)                            cacaATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGtttt                                         <  2:290642/81‑1 (MQ=255)                             acaATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGttttt                                        >  2:290670/1‑81 (MQ=255)                                aTTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGc                                     >  2:290682/1‑81 (MQ=255)                                 tttGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCt                                    <  2:290643/81‑1 (MQ=255)                                     gCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGacc                                <  2:290644/81‑3 (MQ=255)                                           aTTTCGATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGa                          <  1:290645/81‑1 (MQ=255)                                               cGATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATtgg                      >  1:290675/1‑81 (MQ=255)                                               cGATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATtg                       <  1:290646/80‑1 (MQ=255)                                                gATAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATtggc                     >  1:290669/1‑81 (MQ=255)                                                 aTAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAGCAACCGATtggct                    <  2:290647/81‑1 (MQ=255)                                                 aTAACGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATtggct                    <  1:290648/81‑1 (MQ=255)                                                    aCGCGCTCTCCACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATtggctgg                  <  1:290649/80‑1 (MQ=255)                                                             ccACAACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAAc        >  1:290681/1‑81 (MQ=255)                                                               acaACTCCACAACCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTg      >  1:290679/1‑81 (MQ=255)                                                                      cacaACCCAACACCTGGCAACGTCGCTGGTTAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTg  >  2:290680/1‑78 (MQ=255)                                                                          |                                                                          CGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTG  >  NC_000913/1808127‑1808273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑