Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	11,649	A→G	L46L (CTT→CTC)	yaaI ←	DUF2541 domain‑containing protein YaaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,649	0	A	G	100.0%	142.6 / NA	41	L46L (CTT→CTC)	yaaI	DUF2541 domain‑containing protein YaaI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/24);  total (17/24)

TAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGC  >  NC_000913/11572‑11726                                                                              |                                                                              tAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGt                                                                            <  2:2498/81‑1 (MQ=255)   aaCCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGtt                                                                           <  2:2499/80‑1 (MQ=255)    aCCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGttt                                                                          >  2:2561/1‑80 (MQ=255)       gATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAgc                                                                      <  1:2500/81‑1 (MQ=255)       gATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAgc                                                                      >  1:2563/1‑81 (MQ=255)         tGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAgcgc                                                                    <  2:2502/81‑1 (MQ=255)         tGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAgcgc                                                                    <  2:2501/81‑1 (MQ=255)         tGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAgcgc                                                                    >  1:2542/1‑81 (MQ=255)           cACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGcc                                                                   <  1:2503/80‑1 (MQ=255)            aCCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAgcgc                                                                    >  2:2554/1‑78 (MQ=255)            aCCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAgcgc                                                                    >  2:2559/1‑78 (MQ=255)              cGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAgcgc                                                                    >  2:2551/1‑76 (MQ=255)               gCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATc                                                              <  1:2504/81‑1 (MQ=255)                    aCTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTgg                                                         <  1:2505/81‑1 (MQ=255)                          aaTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGt                                                   >  2:2562/1‑81 (MQ=255)                           aTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGt                                                   >  2:2566/1‑80 (MQ=255)                              gCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTAc                                                <  2:2506/80‑1 (MQ=255)                               ccATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTAcgc                                              >  2:2556/1‑81 (MQ=255)                                  tGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCgg                                            >  2:2565/1‑80 (MQ=255)                                  tGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGc                                           <  1:2507/81‑1 (MQ=255)                                    gTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCAt                                         <  2:2508/81‑1 (MQ=255)                                          agagagTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAAt                                   >  1:2560/1‑81 (MQ=255)                                           gagagTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATg                                  >  1:2570/1‑81 (MQ=255)                                           gagagTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATg                                  <  1:2509/81‑1 (MQ=255)                                             gagTTGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGgc                                <  2:2510/81‑1 (MQ=255)                                                ttGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGcc                             <  1:2512/81‑1 (MQ=255)                                                ttGTATGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGcc                             <  1:2511/81‑1 (MQ=255)                                                    aTGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCgag                          >  2:2558/1‑80 (MQ=255)                                                     tGCGTTTCACAATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCgagg                         <  2:2513/80‑1 (MQ=255)                                                              caATGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGt               >  1:2567/1‑81 (MQ=255)                                                                aTGCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCg             <  2:2514/81‑1 (MQ=255)                                                                  gCGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGtt           >  1:2573/1‑81 (MQ=255)                                                                   cGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGttt          <  2:2517/81‑1 (MQ=255)                                                                   cGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGttt          <  2:2516/81‑1 (MQ=255)                                                                   cGACAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGttt          <  2:2515/81‑1 (MQ=255)                                                                     aCAAACAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGc        >  2:2564/1‑81 (MQ=255)                                                                        aaCAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTg     <  1:2518/81‑1 (MQ=255)                                                                         aCAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTgc    <  1:2519/81‑1 (MQ=255)                                                                          cAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTgcg   <  2:2520/81‑1 (MQ=255)                                                                          cAGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTgcg   <  1:2521/81‑1 (MQ=255)                                                                           aGGGAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTgcgc  >  1:730322/1‑81 (MQ=255)                                                                              |                                                                              TAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGC  >  NC_000913/11572‑11726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑