Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,824,185	T→C	V224A (GTG→GCG)	cho →	endonuclease of nucleotide excision repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,824,185	0	T	C	100.0%	59.9 / NA	18	V224A (GTG→GCG)	cho	endonuclease of nucleotide excision repair
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/9);  total (9/9)

CATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGTGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCAAAACTGGCTGTGGCTGGGGGCGG  >  NC_000913/1824109‑1824256                                                                             |                                                                        cATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGgcggcggcg                                                                     >  2:292598/1‑81 (MQ=255)       gAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGaaa                                                               <  1:292570/81‑1 (MQ=255)          cATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGaa                                                            >  1:292596/1‑81 (MQ=255)               gcTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTG‑‑GTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCAcc                                                     <  2:292571/81‑1 (MQ=255)                  aCGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCAccc                                                    >  1:292599/1‑81 (MQ=255)                   cGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCg                                                   >  2:292607/1‑81 (MQ=255)                   cGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCg                                                   >  2:292604/1‑81 (MQ=255)                         cgcCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCGGAAAt                                              <  1:292572/80‑1 (MQ=255)                          gcCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCGGAAATGa                                            >  1:292602/1‑81 (MQ=255)                             aGTCTCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCGGAAATGACTc                                         <  2:292573/81‑1 (MQ=255)                                   tGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCGGAAATGACTCAat                                      <  1:292574/78‑1 (MQ=255)                                           ttGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATattatt                           >  1:292605/1‑81 (MQ=255)                                             gCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTc                          >  2:292603/1‑80 (MQ=255)                                              cGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCa                         <  2:292575/80‑1 (MQ=255)                                                    gtgtgtTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCAAAACtgg                  <  2:292576/81‑1 (MQ=255)                                                    gtgtgtTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCAAAACtg                   <  1:292577/80‑1 (MQ=255)                                                        gtTGGCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCAAAACtggctgt              >  1:292609/1‑81 (MQ=255)                                                            gCCTTGGCAAGGGGCGGCGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCAAAACtggctgtggct          <  1:292578/81‑1 (MQ=255)                                                                    aaGGGGCGGCGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCAAAACTGGCTGTGGCTGGGGGCgg  >  1:292606/1‑81 (MQ=255)                                                                             |                                                                        CATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGTGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCAAAACTGGCTGTGGCTGGGGGCGG  >  NC_000913/1824109‑1824256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑