Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,830,943	T→C	Y347C (TAC→TGC)	astC ←	succinylornithine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,830,943	0	T	C	100.0%	45.6 / NA	14	Y347C (TAC→TGC)	astC	succinylornithine transaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/8);  total (6/8)

CCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATT  >  NC_000913/1830866‑1831016                                                                              |                                                                          ccacGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAAt                                                                        <  2:293549/81‑1 (MQ=255)     gTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCgg                                                                     >  1:293569/1‑80 (MQ=255)     gTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCgg                                                                     >  1:293571/1‑80 (MQ=255)          cACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCa                                                               <  1:293550/81‑1 (MQ=255)                             cACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCa                                             >  1:293576/1‑80 (MQ=255)                               cGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAAc                                          <  2:293551/81‑1 (MQ=255)                               cGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAAc                                          <  2:293552/81‑1 (MQ=255)                                 ccTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCt                                        <  1:293553/81‑1 (MQ=255)                                           ccgcTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGaa                              >  1:293587/1‑81 (MQ=255)                                                 ccTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTc                          <  2:293554/79‑1 (MQ=255)                                                  cTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACt                       <  1:293555/81‑1 (MQ=255)                                                                   cTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATGGCGGt      <  2:293556/81‑1 (MQ=255)                                                                    ttGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGt      >  1:293583/1‑80 (MQ=255)                                                                        ccGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGAtt  >  2:293581/1‑80 (MQ=255)                                                                              |                                                                          CCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATT  >  NC_000913/1830866‑1831016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑