Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,832,618	G→C	G64A (GGC→GCC)	xthA →	exodeoxyribonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,832,618	0	G	C	100.0%	79.4 / NA	24	G64A (GGC→GCC)	xthA	exodeoxyribonuclease III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/9);  total (15/9)

CGATATGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCG  >  NC_000913/1832544‑1832693                                                                           |                                                                            cGATATGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTAtgccgtggc                                                                       <  2:293768/81‑1 (MQ=255)   atatGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGgcgc                                                                     >  1:293795/1‑81 (MQ=255)           ccGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGAc                                                              >  1:293803/1‑80 (MQ=255)              cTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCaaa                                                          <  1:293769/81‑1 (MQ=255)              cTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCa                                                            >  1:293816/1‑79 (MQ=255)               tCGAAGAGGCGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCa                                                            >  1:293807/1‑78 (MQ=255)                 gAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCaaa                                                          >  1:293801/1‑78 (MQ=255)                 gAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAagag                                                       <  2:293770/81‑1 (MQ=255)                   agagGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGAc                                                     <  2:293771/81‑1 (MQ=255)                      ggTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGcc                                                  <  1:293772/81‑1 (MQ=255)                      ggTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGcc                                                  >  1:293804/1‑81 (MQ=255)                           cGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTg                                             >  1:293799/1‑81 (MQ=255)                            gAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAGGAGACGCCGATTGc                                            >  1:293815/1‑81 (MQ=255)                                    gCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTc                                    >  1:293809/1‑81 (MQ=255)                                        cAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCgg                                >  2:293810/1‑81 (MQ=255)                                          aCGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCt                              >  2:293805/1‑81 (MQ=255)                                               ttttATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTccc                         <  2:293773/81‑1 (MQ=255)                                                     cACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTgac                   >  2:293813/1‑81 (MQ=255)                                                        gggCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTgacgac                <  1:293774/81‑1 (MQ=255)                                                          gCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGacgacg               >  2:293812/1‑80 (MQ=255)                                                            aGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAag            <  1:293775/81‑1 (MQ=255)                                                               aaGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAgg         >  1:293820/1‑81 (MQ=255)                                                                 ggCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGgcg       >  1:293818/1‑81 (MQ=255)                                                                 ggCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGATTTCCCGGTGACGACGAAGAGgcg       <  2:293776/81‑1 (MQ=255)                                                                      ttATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAgcg  <  2:293777/81‑1 (MQ=255)                                                                           |                                                                            CGATATGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCG  >  NC_000913/1832544‑1832693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑