Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,861,251	C→T	D28N (GAT→AAT)	ydjL ←	putative zinc‑binding dehydrogenase YdjL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,861,251	0	C	T	100.0%	84.0 / NA	24	D28N (GAT→AAT)	ydjL	putative zinc‑binding dehydrogenase YdjL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/13);  total (11/13)

CATCAGAACCGCTATCGACATTGTAGTGCTTCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATCTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTG  >  NC_000913/1861173‑1861325                                                                               |                                                                           catcaGAACCGCTATCGACATTGTAGTGCTTCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACAtttt                                                                          >  2:297435/1‑81 (MQ=255)   tcaGAACCGCTATCGACATTGTAGTGCTTCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTcc                                                                        <  2:297408/81‑1 (MQ=255)                 gACATTGTAGTGCTTCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATggg                                                          >  2:297434/1‑81 (MQ=255)                  aCATTGTAGTGCTTCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGt                                                         >  2:297442/1‑81 (MQ=255)                   cATTGTAGTGCTTCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGtt                                                        <  1:297409/81‑1 (MQ=255)                    aTTGTAGTGCTTCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTtg                                                       <  1:297410/81‑1 (MQ=255)                    aTTGTAGTGCTTCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTtg                                                       <  1:297411/81‑1 (MQ=255)                    aTTGTAGTGCTTCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTtg                                                       <  1:297412/81‑1 (MQ=255)                       gTAGTGCATCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCTCTACATTTTCCGGGCCACACATGGGTTGTgg                                                    >  2:297433/1‑81 (MQ=255)                             cTTCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTTGTGGGACatc                                              <  2:297414/81‑1 (MQ=255)                             cTTCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTTGTGGGACat                                               <  2:297413/80‑1 (MQ=255)                                cATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTTGTGGGACatcaat                                           >  1:297439/1‑81 (MQ=255)                                   gTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAatcat                                        >  2:297430/1‑81 (MQ=255)                                     cTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCt                                      >  2:297440/1‑81 (MQ=255)                                        cgccgcAGATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGt                                   <  1:297415/81‑1 (MQ=255)                                               gATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGTAgccgcc                            <  1:297416/81‑1 (MQ=255)                                               gATTGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGTAgccgc                             >  1:297441/1‑80 (MQ=255)                                                 ttGCCGCGGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGTAGCCGCCaa                          <  2:297417/81‑1 (MQ=255)                                                       cgGCTTTAATTTCAATCACTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGTAGCCGCCAAAGGCCt                    >  2:297437/1‑81 (MQ=255)                                                               aTTTCAATCACTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAAc            <  2:297418/81‑1 (MQ=255)                                                               aTTTCAATCACTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAAc            >  1:297438/1‑81 (MQ=255)                                                                     aTCACTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGc       >  1:297436/1‑80 (MQ=255)                                                                       cACTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAg    >  1:297450/1‑81 (MQ=255)                                                                         cTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTg  <  1:297419/81‑1 (MQ=255)                                                                         cTACATTTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTg  <  1:297420/81‑1 (MQ=255)                                                                               |                                                                           CATCAGAACCGCTATCGACATTGTAGTGCTTCATGTCTGCGCCGCAGATTGCCGCGGCTTTAATTTCAATCACTACATCTTCCGGGCCACACATGGGTTGTGGGACATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTG  >  NC_000913/1861173‑1861325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑