Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,873,121	G→A	E361K (GAA→AAA)	dgcJ →	putative diguanylate cyclase DgcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,873,121	0	G	A	100.0%	41.7 / NA	13	E361K (GAA→AAA)	dgcJ	putative diguanylate cyclase DgcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/4);  total (9/4)

GTTTATACCAAAATGTGTCGCGAGAAAATATTAGTGATGCGATGACTGGACTGTATAATCGCAAAATTTTAACCCCTGAACTGGAGCAGCGGTTGCAGAAACTGGTGCAATCCGGTTCTTCGGTGATGTTTATTGCTATT  >  NC_000913/1873044‑1873183                                                                              |                                                               gTTTATACCAAAATGTGTCGCGAGAAAATATTAGTGATGCGATGACTGGACTGTATAATCGCAAAATTTTAACCCCTAAAc                                                             >  1:298962/1‑81 (MQ=255) gTTTATACCAAAATGTGTCGCGAGAAAATATTAGTGATGCGATGACTGGACTGTATAATCGCAAAATTTTAACCCCTAAAc                                                             >  2:298961/1‑81 (MQ=255)        ccAAAATGTGTCGCGAGAAAATATTAGTGATGCGATGACTGGACTGTATAATCGCAAAATTTTAACCCCTAAACTGGagca                                                      <  1:298943/81‑1 (MQ=255)         cAAAATGTGTCGCGAGAAAATATTAGTGATGCGATGACTGGACTGTATAATCGCAAAATTTTAACCCCTAAACTGGagcag                                                     >  1:298967/1‑81 (MQ=255)                 gtCGCGAGAAAATATTAGTGATGCGATGACTGGACTGTATAATCGCAAAATTTTAACCCCTAAACTGGAGCAGCGGTTg                                               >  1:298951/1‑79 (MQ=255)                 gtCGCGAGAAAATATTAGTGATGCGATGACTGGACTGTATAATCGCAAAATTTTAACCCCTAAACTGGAGCAGCGGTTg                                               >  2:298963/1‑79 (MQ=255)                    gcgAGAAAATATTAGTGATGCGATGACTGGACTGTATAATCGCAAAATTTTAACCCCTAAACTGGAGCAGCGGTTGCAGa                                           >  1:298970/1‑80 (MQ=255)                           aaTATTAGTGATGCGATGACTGGACTGTATAATCGCAAAATTTTAACCCCTAAACTGGAGCAGCGGTTGCAGAAACTGGTg                                   <  2:298944/81‑1 (MQ=255)                                    gATGCGATGACTGGACTGTATAATCGCAAAATTTTAACCCCTAAACTGGAGCAGCGGTTGCAGAAACTGGTGCAATCCGGt                          >  1:298968/1‑81 (MQ=255)                                           tGACTGGACTGTATAATCGCAAAATTTTAACCCCTAAACTGGAGCAGCGGTTGCAGAAACTGGTGCAATCCGGTTCTTCgg                   <  2:298945/81‑1 (MQ=255)                                            gactggactgTATAATCGCAAAATTTTAACCCCTAAACTGGAGCAGCGGTTGCAGAAACTGGTGCAATCCGGTTCTTCGGt                  >  2:298971/1‑81 (MQ=255)                                            gactggactgTATAATCGCAAAATTTTAACCCCTAAACTGGAGCAGCGGTTGCAGAAACTGGTGCAATCCGGTTCTTCGGt                  >  2:298973/1‑81 (MQ=255)                                                             gCAAAATTTTAACCCCTAAACTGGAGCAGCGGTTGCAGAAACTGGTGCAATCCGGTTCTTCGGTGATGTTTATTGCTAtt  <  2:298946/80‑1 (MQ=255)                                                                              |                                                               GTTTATACCAAAATGTGTCGCGAGAAAATATTAGTGATGCGATGACTGGACTGTATAATCGCAAAATTTTAACCCCTGAACTGGAGCAGCGGTTGCAGAAACTGGTGCAATCCGGTTCTTCGGTGATGTTTATTGCTATT  >  NC_000913/1873044‑1873183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑