Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,878,986	A→G	intergenic (+38/+21)	yoaJ → / ← yeaQ	uncharacterized protein YoaJ/PF04226 family protein YeaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,878,986	0	A	G	100.0%	37.2 / NA	12	intergenic (+38/+21)	yoaJ/yeaQ	uncharacterized protein YoaJ/PF04226 family protein YeaQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/4);  total (8/4)

TCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACG  >  NC_000913/1878917‑1879058                                                                      |                                                                         tCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGc                                                                <  1:299723/80‑1 (MQ=255)            aCTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGt                                                     >  2:299749/1‑80 (MQ=255)                aGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACt                                                <  2:299724/81‑1 (MQ=255)                     ggATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACtttt                                             <  2:299725/79‑1 (MQ=255)                         ggtggtAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCtt                                       >  2:299736/1‑81 (MQ=255)                                  gTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGa                               >  1:299742/1‑80 (MQ=255)                                          tAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGATAACTTTTAATCTTCCTGTAGATAAATAGCa                      >  1:299750/1‑81 (MQ=255)                                            aaaaaTAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCAc                     >  1:299743/1‑80 (MQ=255)                                            aaaaaTAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCAc                     <  2:299726/80‑1 (MQ=255)                                                     aaaGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCa           >  2:299741/1‑81 (MQ=255)                                                         gCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCaa       >  1:299739/1‑81 (MQ=255)                                                              cTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACg  >  1:299738/1‑81 (MQ=255)                                                                      |                                                                         TCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACG  >  NC_000913/1878917‑1879058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑