Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	207,668	C→T	A848V (GCC→GTC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	207,668	0	C	T	100.0%	121.9 / NA	34	A848V (GCC→GTC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/20);  total (14/20)

ATTTCCACGTCAACGACGACGGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAA  >  NC_000913/207590‑207741                                                                               |                                                                          aTTTCCACGTCAACGACGACGGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTca                                                                         >  2:44879/1‑81 (MQ=255)    tCCACGTCAACGACGACGGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTcatc                                                                       <  2:44823/80‑1 (MQ=255)    tCCACGTCAACGACGACGGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTcatc                                                                       <  2:44822/80‑1 (MQ=255)     ccACGTCAACGACGACGGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGtcatca                                                                      <  2:44824/80‑1 (MQ=255)     ccACGTCAACGACGACGGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTcatcat                                                                     >  1:44886/1‑81 (MQ=255)      cACGTCAACGACGACGGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCatcatc                                                                    <  1:44825/81‑1 (MQ=255)         gTCAACGACGACGGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCg                                                                   >  2:44867/1‑79 (MQ=255)            aaCGACGACGGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGcc                                                              >  2:44866/1‑81 (MQ=255)            aaCGACGACGGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGc                                                               <  2:44826/80‑1 (MQ=255)               gacgacgGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGt                                                           <  2:44827/81‑1 (MQ=255)                  gacgGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGtaat                                                        <  1:44828/81‑1 (MQ=255)                     ggCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATaaa                                                     <  1:44830/81‑1 (MQ=255)                     ggCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATaaa                                                     <  1:44829/81‑1 (MQ=255)                       cGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAgg                                                   <  1:44831/81‑1 (MQ=255)                          aaTCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAggcgg                                                >  2:44877/1‑81 (MQ=255)                              gtgtATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTAc                                            <  2:44832/81‑1 (MQ=255)                               tgtATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACt                                           <  2:44833/81‑1 (MQ=255)                                gtATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACtt                                          >  2:44874/1‑81 (MQ=255)                                 tATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACtt                                          <  2:44835/80‑1 (MQ=255)                                           gcgcGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGt                               <  2:44836/81‑1 (MQ=255)                                             gcgATCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGttt                             >  2:44876/1‑81 (MQ=255)                                                aTCAAAGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGAt                          >  2:44882/1‑81 (MQ=255)                                                   aaaGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGAtctc                       >  1:44885/1‑81 (MQ=255)                                                   aaaGGGGTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGAtctc                       >  2:44883/1‑81 (MQ=255)                                                      ggggTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTgc                    >  2:44878/1‑81 (MQ=255)                                                      ggggTCGGTGAAGGTCCGATTGAGGTCATCATCGAAACCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTgc                    <  1:44837/81‑1 (MQ=255)                                                         gTCGGTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGcc                 <  1:44838/81‑1 (MQ=255)                                                            ggTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCg               >  2:44884/1‑80 (MQ=255)                                                            ggTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGt              <  2:44839/81‑1 (MQ=255)                                                             gTGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTa             <  1:44840/81‑1 (MQ=255)                                                              tGAAGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTAc            >  2:44887/1‑81 (MQ=255)                                                                aaGGTCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTAcc           <  1:44841/80‑1 (MQ=255)                                                                    tCCGATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGacac      <  1:44842/81‑1 (MQ=255)                                                                       gATTGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCaa   <  1:44843/81‑1 (MQ=255)                                                                          tGAGGTCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCaaa  >  2:44893/1‑79 (MQ=255)                                                                               |                                                                          ATTTCCACGTCAACGACGACGGCGAAATCGTGTATGGTATTGGCGCGATCAAAGGGGTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAA  >  NC_000913/207590‑207741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑