Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,070	T→C	K155K (AAA→AAG)	proQ ←	RNA chaperone ProQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,070	0	T	C	100.0%	119.1 / NA	34	K155K (AAA→AAG)	proQ	RNA chaperone ProQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/12);  total (22/12)

AGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTC  >  NC_000913/1914992‑1915147                                                                               |                                                                              aGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGctt                                                                             <  2:304549/81‑1 (MQ=255)   aaaCCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTAc                                                                           <  1:304550/81‑1 (MQ=255)      ccGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGtt                                                                        >  1:304586/1‑81 (MQ=255)      ccGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGtt                                                                        <  2:304551/81‑1 (MQ=255)                       tcGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTgcgg                                                       >  2:304594/1‑81 (MQ=255)                        cgcgAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTgc                                                         <  2:304552/78‑1 (MQ=255)                         gcgAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTgcggcg                                                     >  1:304593/1‑81 (MQ=255)                          cgAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCggcgt                                                    <  2:304553/81‑1 (MQ=255)                           gAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCggcgtg                                                   >  2:304595/1‑81 (MQ=255)                             ggTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCggcgtggc                                                 >  1:304575/1‑81 (MQ=255)                                  ttttACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGt                                            >  1:304581/1‑81 (MQ=255)                                  ttttACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGt                                            >  1:304587/1‑81 (MQ=255)                                   tttACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTc                                           >  1:304598/1‑81 (MQ=255)                                               ggCGCTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTg                               >  1:304580/1‑81 (MQ=255)                                                gcgcTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGc                              <  1:304554/81‑1 (MQ=255)                                                gcgcTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGc                              >  2:304583/1‑81 (MQ=255)                                                   cTTTCTCTACCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGtt                           <  1:304555/81‑1 (MQ=255)                                                           aCCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcgc                   >  2:304591/1‑81 (MQ=255)                                                           aCCGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcgc                   >  1:304588/1‑81 (MQ=255)                                                            ccGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggggcg                  >  1:304601/1‑81 (MQ=255)                                                            ccGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcgcg                  >  1:304590/1‑81 (MQ=255)                                                            ccGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcgc                   <  1:304557/80‑1 (MQ=255)                                                            ccGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggc                     <  2:304556/78‑1 (MQ=255)                                                            ccGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggc                     <  2:304558/78‑1 (MQ=255)                                                             cGGCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcgcgg                 >  2:304579/1‑81 (MQ=255)                                                              ggCTTTTGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGt                <  2:304559/81‑1 (MQ=255)                                                                 ttttGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCg             >  1:304599/1‑81 (MQ=255)                                                                  tttGCGCACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGt            >  2:304569/1‑81 (MQ=255)                                                                     gcgcACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGtctt         >  1:304597/1‑81 (MQ=255)                                                                       gcACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGtcttct       >  1:304607/1‑81 (MQ=255)                                                                       gcACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGC‑TGCGTTCGCGGCGCGGTGCGTcttctt      >  2:304584/1‑81 (MQ=255)                                                                        cACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTcttctt      >  1:304589/1‑81 (MQ=255)                                                                        cACGAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTcttctt      <  2:304560/81‑1 (MQ=255)                                                                           gAGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCttctttct   >  1:304613/1‑81 (MQ=255)                                                                            aGGCTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTtctc  <  1:304561/81‑1 (MQ=255)                                                                               |                                                                              AGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTC  >  NC_000913/1914992‑1915147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑