Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,916,632	T→C	V125V (GTT→GTC)	yebS →	intermembrane transport protein YebS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,916,632	0	T	C	100.0%	84.8 / NA	25	V125V (GTT→GTC)	yebS	intermembrane transport protein YebS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/14);  total (11/14)

CGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTTATTGGTGCCCCGCTCATTCTGGTGACCTCCATAGCTTATTTATGGTTTGGTAACCGACTGGGAATGAATTTACGT  >  NC_000913/1916560‑1916707                                                                         |                                                                            cGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGc                                                                     <  2:304809/81‑1 (MQ=255) cGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGc                                                                     <  2:304810/81‑1 (MQ=255)  gctATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGcc                                                                    >  1:304839/3‑81 (MQ=255)  gTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGcc                                                                    >  1:304838/1‑81 (MQ=255)  gTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGcc                                                                    <  1:304811/81‑1 (MQ=255)    tATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGcccc                                                                  >  1:304837/1‑81 (MQ=255)     aTGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCg                                                                 >  1:304841/1‑81 (MQ=255)        cAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTc                                                              <  2:304813/81‑1 (MQ=255)        cAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTc                                                              <  2:304812/81‑1 (MQ=255)            gCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTc                                                          <  1:304814/81‑1 (MQ=255)              aTCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTCTg                                                        <  1:304815/81‑1 (MQ=255)                     aaaTGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTCTGGTGACCt                                                 <  2:304816/81‑1 (MQ=255)                       aTGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTCTGGTGACCTcc                                               <  2:304817/81‑1 (MQ=255)                         gACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTCTGGTGACCTCCa                                              >  2:304847/1‑80 (MQ=255)                          aCCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTCTGGTGACCTCCAt                                             <  2:304818/80‑1 (MQ=255)                                 aGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTCTGGTGACCTCCATAGCttatt                                     >  1:304848/1‑81 (MQ=255)                                 aGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTCTGGTGACCTCCATAGCttatt                                     >  1:304842/1‑81 (MQ=255)                                  gggCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTCTGGTGACCTCCATAGCttattt                                    <  2:304819/81‑1 (MQ=255)                                   ggCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTCTGGTGACCTCCATAGCttattta                                   >  1:304832/1‑81 (MQ=255)                                              aaCGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTCTGGTGACCTCCATAGCTTATTTATGGTTTGGTaa                        >  1:304845/1‑81 (MQ=255)                                                cGGGGTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTCTGGTGACCTCCATAGCTTATTTATGGTTTGGTAAcc                      >  1:304851/1‑81 (MQ=255)                                                 ggggTCGATGGTCTTTTTCTGCGTCATTGGTGCCCCGCTCATTCTGGTGACCTCCATAGCTTATTTATGGTTTGGTAAcc                      >  2:304852/1‑80 (MQ=255)                                                                tttCTGCGTCATTGGTGCCCCGCTCATTCTGGTGACCTCCATAGCTTATTTATGGTTTGGTAACCGACTGGGAATGAAtt       <  2:304820/80‑1 (MQ=255)                                                                      cGTCATTGGTGCCCCGCTCATTCTGGTGACCTCCATAGCTTATTTATGGTTTGGTAACCGACTGGGAATGAATTTACg   <  1:304821/78‑1 (MQ=255)                                                                      cGTCATTGGTGCCCCGCTCATTCTGGTGACCTCCATAGCTTATTTATGGTTTGGTAACCGACTGGGAATGAATTTACGt  <  1:304822/79‑1 (MQ=255)                                                                         |                                                                            CGTTATGCAAGGCATCTGGCAAATGACCAAACAGGGCGATGCGATAACGGGGTCGATGGTCTTTTTCTGCGTTATTGGTGCCCCGCTCATTCTGGTGACCTCCATAGCTTATTTATGGTTTGGTAACCGACTGGGAATGAATTTACGT  >  NC_000913/1916560‑1916707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑