Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,925,648	G→A	C70Y (TGT→TAT)	yobB →	putative carbon‑nitrogen hydrolase family protein YobB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,925,648	0	G	A	100.0%	115.2 / NA	32	C70Y (TGT→TAT)	yobB	putative carbon‑nitrogen hydrolase family protein YobB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/19);  total (13/19)

GCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGG  >  NC_000913/1925571‑1925723                                                                              |                                                                            gCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTttatta                                                                          >  1:306113/1‑81 (MQ=255)     tctttACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGcc                                                                      >  1:306100/1‑81 (MQ=255)     tctttACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGc                                                                       <  1:306067/80‑1 (MQ=255)                        attCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATg                                                    <  2:306068/80‑1 (MQ=255)                          tCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGAc                                                  >  2:306119/1‑80 (MQ=255)                            gcgACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACcat                                               >  2:306118/1‑81 (MQ=255)                              gACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACcatca                                             <  1:306069/81‑1 (MQ=255)                              gACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACcatca                                             >  1:306106/1‑81 (MQ=255)                               aCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACcatcat                                            >  2:306108/1‑81 (MQ=255)                                      cTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGc                                     <  1:306070/81‑1 (MQ=255)                                       ttCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGcc                                    >  2:306111/1‑81 (MQ=255)                                         ccTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCt                                   <  2:306072/80‑1 (MQ=255)                                         ccTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCt                                   <  1:306071/80‑1 (MQ=255)                                          cTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCtt                                  <  2:306073/80‑1 (MQ=255)                                          cTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTc                                 >  2:306123/1‑81 (MQ=255)                                          cTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCACCATTGCCGGCCTTc                                 >  1:306116/1‑81 (MQ=255)                                              cccACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGt                             <  1:306074/81‑1 (MQ=255)                                                 aCCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGa                          <  2:306075/81‑1 (MQ=255)                                                  cccGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGaa                         >  2:306109/1‑81 (MQ=255)                                                          ctcACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGa                 >  2:306105/1‑81 (MQ=255)                                                           tcACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATaa                    <  2:306077/77‑1 (MQ=255)                                                           tcACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACg                  <  2:306076/79‑1 (MQ=255)                                                           tcACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACg                  <  1:306079/79‑1 (MQ=255)                                                           tcACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACg                  <  1:306078/79‑1 (MQ=255)                                                            cACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATc               >  2:306115/1‑81 (MQ=255)                                                             aCTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGAt                <  2:306080/79‑1 (MQ=255)                                                                 ttAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCttt          <  1:306081/81‑1 (MQ=255)                                                                 ttAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCt            <  1:306082/79‑1 (MQ=255)                                                                      cccGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCg     <  2:306083/81‑1 (MQ=255)                                                                        cGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTg   >  1:306121/1‑81 (MQ=255)                                                                        cGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTg   <  1:306084/81‑1 (MQ=255)                                                                         gCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTgg  <  2:306085/81‑1 (MQ=255)                                                                          cTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTgg  >  2:306114/1‑80 (MQ=255)                                                                              |                                                                            GCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGG  >  NC_000913/1925571‑1925723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑