Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,928,777	A→G	D21D (GAT→GAC)	ptrB ←	oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,928,777	0	A	G	100.0%	43.9 / NA	14	D21D (GAT→GAC)	ptrB	oligopeptidase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/5);  total (9/5)

AACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTT  >  NC_000913/1928704‑1928848                                                                          |                                                                        aaCTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATgc                                                                  >  1:306536/1‑81 (MQ=255)      ttttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTa                                                             >  2:306529/1‑81 (MQ=255)          cTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGc                                                         >  2:306531/1‑81 (MQ=255)                gCAGGTCGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGCTGCGCGTATCGCCATGat                                                   >  2:306532/1‑80 (MQ=255)                gCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGa                                                    <  2:306499/80‑1 (MQ=255)                    gTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGt                                               <  2:306500/81‑1 (MQ=255)                     tAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTc                                              <  2:306501/81‑1 (MQ=255)                         ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATgg                                          >  2:306524/1‑81 (MQ=255)                                        gagaACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcggg                           >  2:306525/1‑81 (MQ=255)                                        gagaACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcggg                           >  2:306545/1‑81 (MQ=255)                                                     gTCCCGCAGCCAGTAGTGATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAg              >  1:306544/1‑81 (MQ=255)                                                        ccGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCa            <  1:306502/80‑1 (MQ=255)                                                           cAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGtt        <  2:306503/81‑1 (MQ=255)                                                                 gtagtaATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATGGtt  >  2:306534/1‑81 (MQ=255)                                                                          |                                                                        AACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTT  >  NC_000913/1928704‑1928848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑