Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	208,192	T→A	S1023T (TCC→ACC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	208,192	0	T	A	100.0%	68.4 / NA	20	S1023T (TCC→ACC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/11);  total (9/11)

CGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCG  >  NC_000913/208115‑208262                                                                              |                                                                       cGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTAcc                                                                      <  1:44936/80‑1 (MQ=255) cGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCg                                                                     <  2:44937/81‑1 (MQ=255)      cTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGggcgg                                                                >  1:44974/1‑81 (MQ=255)         gttgttGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCt                                                              <  1:44939/80‑1 (MQ=255)         gttgttGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTg                                                             >  1:44975/1‑81 (MQ=255)              tgCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGTAGt                                                        >  2:44973/1‑81 (MQ=255)                    gcgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtgtt                                                  >  2:44958/1‑81 (MQ=255)                      gggTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtgttgt                                                <  2:44940/81‑1 (MQ=255)                       ggTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATgttgtt                                               <  1:44941/81‑1 (MQ=255)                         ttATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTa                                             <  2:44942/81‑1 (MQ=255)                           aTGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACt                                           >  2:44976/1‑81 (MQ=255)                                     aGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTg                                  >  2:44981/1‑80 (MQ=255)                                            cAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATa                          <  2:44943/81‑1 (MQ=255)                                                        tATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCt              <  2:44944/81‑1 (MQ=255)                                                              cACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGaaa         >  1:44983/1‑80 (MQ=255)                                                              cACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAACTGCTgg            <  2:44945/77‑1 (MQ=255)                                                               aCGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGaaaaa       <  2:44946/81‑1 (MQ=255)                                                               aCGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGaaaaa       <  2:44947/81‑1 (MQ=255)                                                                    ggATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCg  >  2:44978/1‑81 (MQ=255)                                                                    ggATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCg  >  2:44980/1‑81 (MQ=255)                                                                              |                                                                       CGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCG  >  NC_000913/208115‑208262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑