Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,947,941	C→T	A71T (GCA→ACA)	yebC ←	putative transcriptional regulator YebC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,947,941	0	C	T	100.0%	77.8 / NA	22	A71T (GCA→ACA)	yebC	putative transcriptional regulator YebC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/16);  total (6/16)

CCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTT  >  NC_000913/1947871‑1948016                                                                       |                                                                            ccgccAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGTAATTGcgcg                                                                    <  1:309159/80‑1 (MQ=255) ccgccAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGTAATTGcgcg                                                                    <  1:309157/80‑1 (MQ=255) ccgccAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGTAATTGcgcg                                                                    <  2:309158/80‑1 (MQ=255)            gTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGTAATTGCGCGGTTCAgtgtgt                                                         >  2:309193/1‑80 (MQ=255)            gTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGTAATTGCGCGGTTCAGTGTGTc                                                        <  1:309160/81‑1 (MQ=255)                            atgGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATgttgtt                                         <  2:309161/80‑1 (MQ=255)                             tgGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCAtgttgt                                          >  2:309194/1‑78 (MQ=255)                                 ttCCATGTTTGCATCATCATCACCGCCCACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAg                                   >  2:309197/1‑81 (MQ=255)                                        tttGCATCATCATCACCGCCCACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCttt                             <  1:309162/80‑1 (MQ=255)                                        tttGCATCATCATCACCGCCCACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCttt                             <  2:309163/80‑1 (MQ=255)                                          tGCATCATCATCACCGCCCACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTAt                           <  2:309165/80‑1 (MQ=255)                                          tGCATCATCATCACCGCCCACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTAt                           <  1:309164/80‑1 (MQ=255)                                            catcatcatCACCGCCCACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCa                        <  2:309166/81‑1 (MQ=255)                                                atcatcaCCGCCCACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCAccgc                    >  1:309195/1‑81 (MQ=255)                                                atcatcaCCGCCCACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCAccgc                    <  1:309167/81‑1 (MQ=255)                                                      aCCGCCCACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCcgcac               <  2:309168/80‑1 (MQ=255)                                                          cccACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGa          >  1:309196/1‑81 (MQ=255)                                                           ccACACCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGAc         <  2:309169/81‑1 (MQ=255)                                                             acacCACGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGAcgc       <  2:309170/81‑1 (MQ=255)                                                              caccacGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGAcgcg      <  2:309171/81‑1 (MQ=255)                                                                 cacGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGt   >  2:309200/1‑81 (MQ=255)                                                                  acGTGTAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGtt  <  2:309172/81‑1 (MQ=255)                                                                       |                                                                            CCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTT  >  NC_000913/1947871‑1948016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑