Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,962,324	T→C	V118A (GTT→GCT)	yecT →	protein YecT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,962,324	0	T	C	100.0%	48.5 / NA	15	V118A (GTT→GCT)	yecT	protein YecT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/7);  total (8/7)

GCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGC  >  NC_000913/1962250‑1962400                                                                           |                                                                             gCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGa                                                                        >  1:311226/1‑81 (MQ=255)  cTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGAt                                                                       <  1:311199/81‑1 (MQ=255)          aTCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGa                                                               <  1:311200/81‑1 (MQ=255)               aGCGAGCCTGGCTGGAGTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTAAGGATTTAAGTGACCCtt                                                          <  2:311201/81‑1 (MQ=255)                gCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTa                                                         <  1:311202/81‑1 (MQ=255)                                   aGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGct                                       <  2:311203/80‑1 (MQ=255)                                      gACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCa                                   >  2:311220/1‑81 (MQ=255)                                           aGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTAt                              >  1:311225/1‑81 (MQ=255)                                                              tCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATAcg           >  2:311229/1‑81 (MQ=255)                                                               cAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATAcgc          >  2:311224/1‑81 (MQ=255)                                                               cAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATAcg           <  2:311204/80‑1 (MQ=255)                                                                aaaTGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATAcgc          >  1:311230/1‑80 (MQ=255)                                                                       gACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGc  <  1:311205/81‑1 (MQ=255)                                                                       gACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGc  >  1:311223/1‑81 (MQ=255)                                                                        aCGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGc  >  1:311227/1‑80 (MQ=255)                                                                           |                                                                             GCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGC  >  NC_000913/1962250‑1962400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑