Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,968,362	T→C	K47R (AAA→AGA)	cheB ←	protein‑glutamate methylesterase/protein glutamine deamidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,968,362	0	T	C	100.0%	77.8 / NA	23	K47R (AAA→AGA)	cheB	protein‑glutamate methylesterase/protein glutamine deamidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/14);  total (9/14)

ATTAATTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTGTTGAT  >  NC_000913/1968287‑1968435                                                                            |                                                                          aTTAATTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCtt                                                                       >  2:312141/1‑80 (MQ=255)    aaTTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAAt                                                                    >  2:312137/1‑80 (MQ=255)     aTTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATc                                                                   >  2:312129/1‑80 (MQ=255)     aTTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCa                                                                  <  2:312098/81‑1 (MQ=255)           cGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCAAGTCAc                                                            >  2:312132/1‑81 (MQ=255)            gAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCAAGTCAcg                                                           <  1:312099/81‑1 (MQ=255)            gAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCAAGTCAc                                                            <  1:312100/80‑1 (MQ=255)                  aTCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCAAGTCACGCGCGAc                                                     >  2:312135/1‑81 (MQ=255)                          cgtccATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGAt                                             <  1:312101/81‑1 (MQ=255)                           gtccATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATc                                            <  1:312102/81‑1 (MQ=255)                               aTCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGgc                                        <  2:312103/81‑1 (MQ=255)                                 ccGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGgcgc                                      >  1:312140/1‑81 (MQ=255)                                    cgGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGt                                   <  2:312104/81‑1 (MQ=255)                                     ggCATTTCAACATCCAGCGTAAGCACATCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTc                                  <  2:312105/81‑1 (MQ=255)                                      gCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCg                                 <  2:312106/81‑1 (MQ=255)                                           tCAACATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGccac                             <  1:312107/80‑1 (MQ=255)                                              aCATCCAGCGTCAGCACATCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCAtt                         <  1:312108/81‑1 (MQ=255)                                                   cAGCATCAGCACATCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCAt                    <  1:312109/81‑1 (MQ=255)                                                       gTCAGCACATCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCg                >  1:312152/1‑81 (MQ=255)                                                            cacaTCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATg           <  2:312110/81‑1 (MQ=255)                                                              caTCGGGATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGc         <  1:312111/81‑1 (MQ=255)                                                                   ggATTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTGtt     >  2:312147/1‑80 (MQ=255)                                                                     aTTGAATCTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTGTtgat  >  1:312146/1‑81 (MQ=255)                                                                            |                                                                          ATTAATTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTGTTGAT  >  NC_000913/1968287‑1968435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑