Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,993,117	G→A	I530I (ATC→ATT)	uvrC ←	excision nuclease subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,993,117	0	G	A	100.0%	54.0 / NA	16	I530I (ATC→ATT)	uvrC	excision nuclease subunit C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/11);  total (5/11)

TTGACCTTCGCCCGTTTTTTACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGT  >  NC_000913/1993049‑1993192                                                                     |                                                                            ttGACCTTCGCCCGTTTTTTACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAgc                                                                 >  2:315664/1‑81 (MQ=255)   gACCTTCGCCCGTTTTTTACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAgcg                                                                <  1:315645/80‑1 (MQ=255)         cGCCCGTTTTTTACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGt                                                           <  2:315647/79‑1 (MQ=255)         cGCCCGTTTTTTACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTg                                                          <  1:315646/80‑1 (MQ=255)                    tACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTggcggc                                               <  2:315648/80‑1 (MQ=255)                      cGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCaaa                                            >  2:315668/1‑81 (MQ=255)                              gCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATcc                                    <  1:315649/81‑1 (MQ=255)                                 aaTCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCt                                  >  1:315678/1‑80 (MQ=255)                                 aaTCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTc                                 <  1:315650/81‑1 (MQ=255)                                 aaTCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTc                                 <  1:315651/81‑1 (MQ=255)                                          aTCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCg                         <  1:315652/80‑1 (MQ=255)                                             aTGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTc                     <  1:315653/81‑1 (MQ=255)                                                     cATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAAc              <  1:315655/80‑1 (MQ=255)                                                     cATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAAc              <  2:315654/80‑1 (MQ=255)                                                         gcgAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGt         >  1:315677/1‑81 (MQ=255)                                                                tGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagt  >  1:315672/1‑81 (MQ=255)                                                                     |                                                                            TTGACCTTCGCCCGTTTTTTACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGT  >  NC_000913/1993049‑1993192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑