Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,995,681	T→C	Y9Y (TAT→TAC)	yecU →	protein YecU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,995,681	0	T	C	100.0%	44.9 / NA	14	Y9Y (TAT→TAC)	yecU	protein YecU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/7);  total (7/7)

ATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATA  >  NC_000913/1995610‑1995758                                                                        |                                                                              atatGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTa                                                                      <  2:315986/81‑1 (MQ=255)        aCATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTtat                                                                <  1:315987/80‑1 (MQ=255)              attaaCCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACt                                                          <  2:315988/80‑1 (MQ=255)              attaaCCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTg                                                         <  2:315989/81‑1 (MQ=255)                 aaCCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGcc                                                       >  2:316003/1‑80 (MQ=255)                                 gCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCaaaa                                       >  2:316006/1‑80 (MQ=255)                                    aaaaaCATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAaac                                    >  2:316009/1‑80 (MQ=255)                                       aaCATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAaacaac                                 >  2:316008/1‑80 (MQ=255)                                           ttCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTg                            <  2:315990/81‑1 (MQ=255)                                                 aTAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTAttt                      <  2:315991/81‑1 (MQ=255)                                                      aaTATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGc                 <  1:315992/81‑1 (MQ=255)                                                            tattGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAggt           >  1:316004/1‑79 (MQ=255)                                                            tattGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAggt           >  2:316012/1‑79 (MQ=255)                                                                     ttACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGtata  >  1:315998/1‑81 (MQ=255)                                                                        |                                                                              ATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATA  >  NC_000913/1995610‑1995758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑