Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,007,153	C→T	T333I (ACC→ATC)	amyA →	alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,007,153	0	C	T	100.0%	92.8 / NA	26	T333I (ACC→ATC)	amyA	alpha‑amylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/16);  total (10/16)

TGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGG  >  NC_000913/2007078‑2007230                                                                            |                                                                              tGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAAc                                                                          <  2:317551/81‑1 (MQ=255)      cAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAAGCGTTg                                                                     >  2:317597/1‑81 (MQ=255)      cAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTg                                                                     <  2:317552/81‑1 (MQ=255)       aGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTg                                                                     >  2:317592/1‑80 (MQ=255)          ttttCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAg                                                                 <  2:317553/81‑1 (MQ=255)           tttCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGc                                                                >  2:317595/1‑81 (MQ=255)                 gggTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGa                                                          <  2:317554/81‑1 (MQ=255)                   gTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAg                                                        <  1:317555/81‑1 (MQ=255)                           gTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTc                                                <  1:317556/81‑1 (MQ=255)                           gTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTc                                                <  1:317557/81‑1 (MQ=255)                             ggAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCg                                               >  1:317594/1‑80 (MQ=255)                                    gATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTgg                                       >  1:317593/1‑81 (MQ=255)                                     aTCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGGCATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGt                                      <  2:317558/81‑1 (MQ=255)                                        cTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTa                                   <  1:317559/81‑1 (MQ=255)                                             cACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCg                              <  2:317560/81‑1 (MQ=255)                                              aCGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGc                             <  1:317561/81‑1 (MQ=255)                                                gCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTg                           >  2:317601/1‑81 (MQ=255)                                                gCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTg                           >  2:317591/1‑81 (MQ=255)                                                          tCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCt                 >  1:317599/1‑81 (MQ=255)                                                            gTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCtt                >  2:317598/1‑80 (MQ=255)                                                              tGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAAt             >  2:317596/1‑81 (MQ=255)                                                                ccAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAAttt           <  2:317563/81‑1 (MQ=255)                                                                ccAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAAtt            <  1:317562/80‑1 (MQ=255)                                                                      aCGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTttgttg      <  2:317564/80‑1 (MQ=255)                                                                      aCGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGc     <  2:317565/81‑1 (MQ=255)                                                                        gACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCgg   <  1:317566/81‑1 (MQ=255)                                                                         acatCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCggg  <  1:317567/81‑1 (MQ=255)                                                                            |                                                                              TGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGG  >  NC_000913/2007078‑2007230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑