Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	215,252	A→G	E140E (GAA→GAG)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	215,252	0	A	G	100.0%	130.9 / NA	37	E140E (GAA→GAG)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/19);  total (18/19)

AAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGAT  >  NC_000913/215175‑215329                                                                              |                                                                              aaaGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTa                                                                             >  1:46429/1‑80 (MQ=255)  aaGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTaaa                                                                           <  1:46395/81‑1 (MQ=255)  aaGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTaaa                                                                           <  1:46394/81‑1 (MQ=255)   agagCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTaaa                                                                           >  1:46454/1‑80 (MQ=255)     agCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTaaa                                                                           >  2:46427/1‑78 (MQ=255)     agCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAaag                                                                        <  2:46396/81‑1 (MQ=255)        gcAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAaagaag                                                                     >  1:46437/1‑81 (MQ=255)         cAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAgg                                                                    <  2:46397/81‑1 (MQ=255)          aGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGa                                                                   <  2:46398/81‑1 (MQ=255)            gCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAat                                                                 <  2:46399/81‑1 (MQ=255)                      aaaGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGaa                                                       <  1:46400/81‑1 (MQ=255)                      aaaGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGa                                                        >  1:46436/1‑80 (MQ=255)                               aaaTCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGAt                                              <  1:46401/81‑1 (MQ=255)                                  tCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGt                                           <  1:46402/81‑1 (MQ=255)                                     aGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGAc                                        <  2:46403/81‑1 (MQ=255)                                      gCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGa                                         >  1:46438/1‑79 (MQ=255)                                        gTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGc                                     >  2:46446/1‑81 (MQ=255)                                        gTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGc                                     <  1:46404/81‑1 (MQ=255)                                        gTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGc                                     >  1:46451/1‑81 (MQ=255)                                          gAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGa                                   >  1:46443/1‑81 (MQ=255)                                          gAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGa                                   >  1:46445/1‑81 (MQ=255)                                              gCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGc                               <  1:46405/81‑1 (MQ=255)                                                gATGCGCGGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTg                             >  2:46448/1‑81 (MQ=255)                                                      cgGCAAAGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCa                       <  2:46406/81‑1 (MQ=255)                                                          aaaGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCct                   <  1:46407/81‑1 (MQ=255)                                                            aGTGCGCAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCctct                 >  2:46452/1‑81 (MQ=255)                                                                 gcAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTAct            >  1:46457/1‑81 (MQ=255)                                                                 gcAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTAct            >  1:46441/1‑81 (MQ=255)                                                                  cAGCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTActc           <  1:46408/81‑1 (MQ=255)                                                                    gCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTg         <  1:46409/81‑1 (MQ=255)                                                                    gCGGTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTg         <  2:46410/81‑1 (MQ=255)                                                                      ggTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGAt       >  1:46442/1‑81 (MQ=255)                                                                      ggTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGAt       <  1:46411/81‑1 (MQ=255)                                                                       gTCGGGAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATg      >  1:46472/1‑81 (MQ=255)                                                                          gggAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGa   <  1:46412/81‑1 (MQ=255)                                                                          gggAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGa   >  2:46450/1‑81 (MQ=255)                                                                           ggAGTAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGAt  >  2:46462/1‑81 (MQ=255)                                                                              |                                                                              AAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGAT  >  NC_000913/215175‑215329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑