Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,015,409	A→G	E177G (GAA→GGA)	fliG →	flagellar motor switch protein FliG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,015,409	0	A	G	100.0%	73.6 / NA	22	E177G (GAA→GGA)	fliG	flagellar motor switch protein FliG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/14);  total (8/14)

TGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAAT  >  NC_000913/2015337‑2015484                                                                         |                                                                            tGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTg                                                                      >  2:318710/1‑80 (MQ=255)       acgacgTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGca                                                               <  2:318678/81‑2 (MQ=255)       acgacgTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCt                                                               <  1:318676/81‑1 (MQ=255)       acgacgTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCt                                                               <  2:318677/81‑1 (MQ=255)        cgacgTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCtt                                                              <  2:318680/81‑1 (MQ=255)        cgacgTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCtt                                                              <  2:318679/81‑1 (MQ=255)            gTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTc                                                          <  1:318681/81‑1 (MQ=255)                        aTCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAAt                                              >  1:318717/1‑81 (MQ=255)                                   tGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAgcgc                                     <  2:318682/79‑1 (MQ=255)                                     gcggcgTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCgcagc                                  <  2:318683/80‑1 (MQ=255)                                       ggcgTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCaa                                >  1:318709/1‑80 (MQ=255)                                         cgTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAAt                             <  2:318684/81‑1 (MQ=255)                                          gTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATg                            >  2:318713/1‑81 (MQ=255)                                           tGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATgg                           >  1:318711/1‑81 (MQ=255)                                           tGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATgg                           <  2:318685/81‑1 (MQ=255)                                            gCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATggg                          >  1:318712/1‑81 (MQ=255)                                                 cagccGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGgcggcg                     <  1:318687/81‑1 (MQ=255)                                                 cagccGCGCTGGCGGAGCTGACCGGAGTAATGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGgcggcg                     <  1:318686/81‑1 (MQ=255)                                                    ccGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTga                  <  2:318688/81‑1 (MQ=255)                                                        gcTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAAc              <  1:318689/81‑1 (MQ=255)                                                                    gACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAAt  >  1:318728/1‑81 (MQ=255)                                                                    gACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAAt  >  2:318720/1‑81 (MQ=255)                                                                         |                                                                            TGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAAT  >  NC_000913/2015337‑2015484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑