Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,021,488	G→T	R134L (CGC→CTC)	fliN →	flagellar motor switch protein FliN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,021,488	0	G	T	100.0%	74.7 / NA	22	R134L (CGC→CTC)	fliN	flagellar motor switch protein FliN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/11);  total (11/11)

GAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGT  >  NC_000913/2021415‑2021565                                                                          |                                                                              gAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGc                                                                        <  2:319715/81‑1 (MQ=255)  aaGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGcc                                                                       <  1:319716/81‑1 (MQ=255)   aGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCg                                                                      <  2:319717/81‑1 (MQ=255)    gtggtgGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGt                                                                     <  1:319718/81‑1 (MQ=255)    gtggtgGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGt                                                                     >  1:319753/1‑81 (MQ=255)          gtCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGtga                                                               <  2:319719/81‑1 (MQ=255)             gTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGtgatg                                                             >  1:319758/1‑80 (MQ=255)             gTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGtgatg                                                             >  1:319765/1‑80 (MQ=255)                        atatGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCt                                                 <  2:319720/81‑1 (MQ=255)                              cGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACtgtg                                           >  2:319747/1‑81 (MQ=255)                              cGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACtgtg                                           <  1:319721/81‑1 (MQ=255)                                     aTCACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCtt                                    <  1:319722/81‑1 (MQ=255)                                       cACCGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTcc                                  >  2:319752/1‑81 (MQ=255)                                         ccGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCcgc                                <  2:319723/81‑1 (MQ=255)                                          cGATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCcgcg                               <  1:319724/81‑1 (MQ=255)                                           gATATCATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCgcgc                              >  1:319757/1‑81 (MQ=255)                                                cATTACTCCGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGtt                         >  1:319755/1‑81 (MQ=255)                                                       ccGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTctgctg                  >  2:319759/1‑81 (MQ=255)                                                        cGTCTGAGCGAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCtgctgc                 >  1:319769/1‑81 (MQ=255)                                                           cTGAGCGAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTgcgc               >  2:319771/1‑80 (MQ=255)                                                                 gAATGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCActgct        <  1:319725/81‑1 (MQ=255)                                                                   aTGCGCCTCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCActgctg       >  2:319750/1‑80 (MQ=255)                                                                       gcctccTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGt  >  1:319772/1‑81 (MQ=255)                                                                       gcctccTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGt  >  1:319760/1‑81 (MQ=255)                                                                          |                                                                              GAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGT  >  NC_000913/2021415‑2021565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑