Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,029,902	C→T	R122C (CGC→TGC)	yedA →	putative transporter YedA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,029,902	0	C	T	100.0%	75.6 / NA	22	R122C (CGC→TGC)	yedA	putative transporter YedA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/9);  total (13/9)

GCCGCCGTAGTGGTTGCAACCGTGCCCCTCTTTACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGCGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGAATCATCATGCTCAATAGCGGTGGAAATTTAAGCG  >  NC_000913/2029833‑2029977                                                                      |                                                                            gccgccGTAGTGGTTGCAACCGTGCCCCTCTTTACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGa                                                                   <  1:320918/80‑1 (MQ=255)  ccaccgTAGTGGTTGCAACCGTGCCCCTCTTTACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAAt                                                                 <  2:320919/78‑1 (MQ=255)   cgccgTAGTGGTTGCAACCGTGCCCCTCTTTACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAAtg                                                                >  2:320946/1‑81 (MQ=255)   cgccgTAGTGGTTGCAACCGTGCCCCTCTTTACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAAtg                                                                >  2:320937/1‑81 (MQ=255)    gccgTAGTGGTTGCAACCGTGCCCCTCTTTACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAAtgg                                                               >  1:320942/1‑81 (MQ=255)       gTAGTGGTTGCAACCGTGCCCCTCTTTACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAAtgggtg                                                            >  2:320938/1‑81 (MQ=255)              ttGCAACCGTGCCCCTCTTTACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTg                                                     <  2:320920/81‑1 (MQ=255)                 cAACCGTGCCCCTCTTTACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCa                                                  >  2:320940/1‑81 (MQ=255)                       tGCCCCTCTTTACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGc                                            <  2:320921/81‑1 (MQ=255)                         ccccTCTTTACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCtt                                          <  2:320922/81‑1 (MQ=255)                                ttACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGaa                                   >  2:320943/1‑81 (MQ=255)                                 tACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCgg                                     <  2:320923/78‑1 (MQ=255)                                   cccTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGAatc                                 <  2:320924/80‑1 (MQ=255)                                              aGCCGCCTGTTTGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGAATCATCATGCTCaa                      <  1:320925/80‑1 (MQ=255)                                               gccgccTGTTTGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGAATCATCATGCTCAATa                    <  1:320926/81‑1 (MQ=255)                                                  gccTGTTTGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGAATCATCATGCTCAATAGCg                 >  1:320949/1‑81 (MQ=255)                                                   ccTGTTTGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGAATCATCATGCTCAATAGCgg                >  1:320948/1‑81 (MQ=255)                                                    cTGTTTGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGAATCATCATGCTCAATAGCGGt               >  2:320945/1‑81 (MQ=255)                                                        ttGGCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGAATCATCATGCTCAATAGCGGTGGa            >  2:320961/1‑80 (MQ=255)                                                           gCATTAAAACGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGAATCATCATGCTCAATAGCGGTGGAAAtt        >  1:320950/1‑81 (MQ=255)                                                                 aaaCGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGAATCATCATGCTCAATAGCGGTGGAAATTTAAGCg  >  2:320939/1‑81 (MQ=255)                                                                 aaaCGTGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGAATCATCATGCTCAATAGCGGTGGAAATTTAAGCg  >  1:320941/1‑81 (MQ=255)                                                                      |                                                                            GCCGCCGTAGTGGTTGCAACCGTGCCCCTCTTTACCCTGTGCTTCAGCCGCCTGTTTGGCATTAAAACGCGCAAACTGGAATGGGTGGGTATTGCCATTGGGCTTGCCGGAATCATCATGCTCAATAGCGGTGGAAATTTAAGCG  >  NC_000913/2029833‑2029977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑