Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,062,407	G→A	S306L (TCA→TTA)	ldtA ←	L,D‑transpeptidase ErfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,062,407	0	G	A	100.0%	61.4 / NA	18	S306L (TCA→TTA)	ldtA	L,D‑transpeptidase ErfK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/8);  total (10/8)

GGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACT  >  NC_000913/2062329‑2062478                                                                               |                                                                        ggagCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTaaa                                                                       >  1:325161/1‑81 (MQ=255)        aaTTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTAAACCAGAAc                                                                <  1:325134/81‑1 (MQ=255)                     cTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAgg                                                   >  1:325152/1‑81 (MQ=255)                        cTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCAt                                                >  1:325154/1‑81 (MQ=255)                            cTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTccc                                            >  2:325157/1‑81 (MQ=255)                              aGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGa                                          >  2:325158/1‑81 (MQ=255)                              aGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGa                                          >  2:325155/1‑81 (MQ=255)                              aGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGa                                          >  1:325159/1‑81 (MQ=255)                                cAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGATc                                        <  2:325135/81‑1 (MQ=255)                                        ttGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTg                                 <  1:325136/80‑1 (MQ=255)                                           cTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAAc                             <  2:325138/81‑1 (MQ=255)                                           cTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAAc                             <  1:325137/81‑1 (MQ=255)                                                tAATTACCAACGCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACgcag                         >  2:325167/1‑80 (MQ=255)                                                          cGCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGa              >  1:325163/1‑81 (MQ=255)                                                           gCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGAt             <  2:325140/81‑1 (MQ=255)                                                           gCTCTTAAAACATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGAt             <  1:325139/81‑1 (MQ=255)                                                                 aaaaCATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACAt       >  1:325156/1‑81 (MQ=255)                                                                   aaCATCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCa     <  1:325141/81‑1 (MQ=255)                                                                      aTCTGTCTTAAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAAct  >  1:325170/1‑81 (MQ=255)                                                                               |                                                                        GGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACT  >  NC_000913/2062329‑2062478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑