Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	217,355	A→G	F474S (TTC→TCC)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	217,355	0	A	G	100.0%	128.0 / NA	37	F474S (TTC→TCC)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/20);  total (17/20)

GCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCTGCTCAATCGCCGCAGCTAC  >  NC_000913/217282‑217422                                                                          |                                                                    gCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGgcgc                                                              >  1:46859/1‑81 (MQ=255)  cTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGgcgc                                                              >  2:46854/1‑80 (MQ=255)  cTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGgcgc                                                              >  1:46839/1‑80 (MQ=255)     tACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGa                                                            <  2:46800/79‑1 (MQ=255)     tACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGAt                                                           <  1:46799/80‑1 (MQ=255)         gTTTCCCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGt                                                      >  2:46844/1‑81 (MQ=255)          tttCTCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTc                                                     <  2:46803/81‑1 (MQ=255)          tttCTCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTc                                                     <  2:46802/81‑1 (MQ=255)          tttCTCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTc                                                     <  2:46801/81‑1 (MQ=255)             ctcAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAgg                                                  >  2:46850/1‑81 (MQ=255)                aGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAgg                                                  <  2:46804/78‑1 (MQ=255)                aGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCa                                               >  2:46861/1‑81 (MQ=255)                    aGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATAc                                            >  2:46851/1‑80 (MQ=255)                      ctctTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGAt                                         <  1:46806/81‑1 (MQ=255)                      ctctTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGAt                                         <  2:46805/81‑1 (MQ=255)                           gTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGc                                       <  1:46807/78‑1 (MQ=255)                           gTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCtc                                    >  1:46855/1‑81 (MQ=255)                              cgcgGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCtcgt                                  <  2:46808/80‑1 (MQ=255)                               gcgGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCtcgttc                                >  2:46845/1‑81 (MQ=255)                                 ggAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCtcgttcgt                              <  1:46809/81‑1 (MQ=255)                                  gAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTtcgtc                             <  1:46810/81‑1 (MQ=255)                                   aaGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTtcgtcg                            >  2:46846/1‑81 (MQ=255)                                    aGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTcgtcgt                           <  2:46811/81‑1 (MQ=255)                                     ggATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTtcgtcg                            >  1:46852/1‑79 (MQ=255)                                       aTTTGTGCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGt                        >  1:46856/1‑81 (MQ=255)                                           gtgCATGTTCATCGGCAGAATCGCTACCTGGGCCGGCGCGATAGCGTCAGGCTATACGATGCCTCGTTCGCCGTAGTTCTg                    >  2:46871/1‑81 (MQ=255)                                           gtgCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCt                     <  2:46812/80‑1 (MQ=255)                                            tgCATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCTGc                   >  2:46858/1‑81 (MQ=255)                                              cATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCTGCt                  <  1:46813/80‑1 (MQ=255)                                              cATGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCTGCTc                 <  1:46814/81‑1 (MQ=255)                                                tGTTCATCGGCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCTGCTCaa               <  2:46815/81‑1 (MQ=255)                                                        ggCAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCTGCTCAATcgccg         >  2:46863/1‑79 (MQ=255)                                                         gCATAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCTGCTCAATCGCCGCAg      <  2:46817/81‑1 (MQ=255)                                                         gCAGAGTCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCTGCTCAATCGCCGCAg      <  1:46816/81‑1 (MQ=255)                                                          cAGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCTGCTCAATCGCCGCAGc     >  2:46870/1‑81 (MQ=255)                                                           aGAATCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCTGCTCAATCGCCGCAGCt    <  2:46818/81‑1 (MQ=255)                                                              aTCGCCACCTGGGACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCTGCTCAATCGCCGCAGCTAc  >  2:46857/1‑80 (MQ=255)                                                                          |                                                                    GCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATAGCGTCAGGCCATACGATGCCTCGTTCGTCGTAGTTCTGCTCAATCGCCGCAGCTAC  >  NC_000913/217282‑217422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑