Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,081,891	C→A	G219G (GGG→GGT)	dacD ←	D‑alanyl‑D‑alanine carboxypeptidase DacD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,081,891	0	C	A	100.0%	66.7 / NA	20	G219G (GGG→GGT)	dacD	D‑alanyl‑D‑alanine carboxypeptidase DacD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/10);  total (10/10)

AATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCT  >  NC_000913/2081816‑2081964                                                                            |                                                                          aaTGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGt                                                                       >  2:328032/1‑80 (MQ=255)     agaTTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACgg                                                                  >  2:328038/1‑81 (MQ=255)     agaTTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACgg                                                                  <  2:328006/81‑1 (MQ=255)      gaTTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGt                                                                 >  1:328045/1‑81 (MQ=255)        ttAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGttt                                                               >  2:328047/1‑81 (MQ=255)        ttAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGttt                                                               <  2:328007/81‑1 (MQ=255)                 gCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGTTTTGCTGGGTg                                                      <  2:328008/81‑1 (MQ=255)                  cACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGTTTTGCTGGGTGa                                                     >  2:328040/1‑81 (MQ=255)                   aCCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGTTTTGCTGGGTGAt                                                    <  2:328009/81‑1 (MQ=255)                        aaGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGTTTTGCTGGGTGATACCGt                                               <  1:328010/81‑1 (MQ=255)                         aGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGTTTTGCTGGGTGATACCGtt                                              <  2:328011/81‑1 (MQ=255)                          gTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGTTTTGCTGGGTGATACCGTTc                                             <  1:328012/81‑1 (MQ=255)                              gACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGTTTTGCTGGGTGATACCGTTCCAgg                                         >  1:328044/1‑81 (MQ=255)                                  cGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTgag                                     <  2:328013/81‑1 (MQ=255)                                    ttttCAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGAc                                   >  1:328043/1‑81 (MQ=255)                                        cAGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtttt                               >  2:328051/1‑81 (MQ=255)                                         aGGCCGTCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtttt                               <  2:328014/80‑1 (MQ=255)                                               tCAACATTCATGGTTTTATCCCACAACAAACCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTg                        <  2:328015/81‑1 (MQ=255)                                                                aTCCCACAACAAACCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTc       >  1:328056/1‑81 (MQ=255)                                                                     acaacaAACCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCt  >  1:328049/1‑81 (MQ=255)                                                                            |                                                                          AATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCT  >  NC_000913/2081816‑2081964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑