Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,088,440	A→T	L230* (TTA→TAA)	yeeZ ←	putative epimerase YeeZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,088,440	0	A	T	100.0%	85.6 / NA	24	L230* (TTA→TAA)	yeeZ	putative epimerase YeeZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (10/14);  total (10/14)

GACTGCCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGATGTGTCCGCCT  >  NC_000913/2088369‑2088514                                                                        |                                                                           gACTGCCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCa                                                                   >  2:329016/1‑81 (MQ=255)   cTGCCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATc                                                                 <  1:328987/81‑1 (MQ=255)      ccATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGc                                                              >  1:329019/1‑81 (MQ=255)        aTCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCgg                                                            <  2:328988/81‑1 (MQ=255)         tCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGa                                                           <  2:328989/81‑1 (MQ=255)           aataatCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGAt                                                          <  1:328990/80‑1 (MQ=255)              aatCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGTGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGa                                                       >  1:329012/1‑80 (MQ=255)                 cTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGaaaa                                                    >  2:329015/1‑80 (MQ=255)                    aCCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAAc                                                   <  2:328991/78‑1 (MQ=255)                    aCCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACAt                                                 <  1:328992/80‑1 (MQ=255)                           ccGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACgcgc                                          <  1:328993/80‑1 (MQ=255)                            cGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAg                                        >  2:329022/1‑81 (MQ=255)                            cGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAg                                        >  1:329025/1‑81 (MQ=255)                              cTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAggg                                      >  1:329023/1‑81 (MQ=255)                                gTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGt                                     >  2:329018/1‑80 (MQ=255)                                                aaCTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACata                      <  1:328995/79‑1 (MQ=255)                                                aaCTGCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACata                      <  2:328994/79‑1 (MQ=255)                                                    gCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATAtatta                 <  2:328996/80‑1 (MQ=255)                                                    gCGGAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATAtatta                 >  2:329020/1‑80 (MQ=255)                                                      ggAGGTTCCAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATAtatta                 >  1:329027/1‑78 (MQ=255)                                                             ccAGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGATgtgt        <  1:328997/80‑1 (MQ=255)                                                               aGCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGATGTGTCCg     <  2:328998/81‑1 (MQ=255)                                                                gCCCCAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGATGTGTCCGc    <  2:328999/81‑1 (MQ=255)                                                                  cccAGTTAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGATGTGTCCGCCt  <  1:329000/81‑1 (MQ=255)                                                                        |                                                                           GACTGCCATCAATAATCTTACCTTTGCCGCTGTCCAGACTATTTCTGAACTGCGGAGGTTCCAGCCCCAGTAAACGGGCCATCTGCGGATAGAAAACATTACGCGCAGGGTGAGCTGGCGCACATATATTATAGATGTGTCCGCCT  >  NC_000913/2088369‑2088514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑