Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,103,378	T→C	pseudogene (12/446 nt)	wbbL ←	interrupted rhamnosyltransferase WbbL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,103,378	0	T	C	100.0%	34.2 / NA	11	pseudogene (12/446 nt)	wbbL	interrupted rhamnosyltransferase WbbL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/7);  total (4/7)

GAGTAATTTTTTGATGTAGTCTTCATGTCCGTGGGAAACGATTATTATATATACCATTTCAATGTTCTTCAGTAATAAAATTAACTAGTTCATCAAACCCAACTAATACATTTTCATT  >  NC_000913/2103333‑2103450                                              |                                                                         gAGTAATTTTTTGATGTAGTCTTCATGTCCGTGGGAAACGATTATCATATATACCATTTCAATGTTCTTCAGTAATaaaa                                        <  2:331308/80‑1 (MQ=255)  aGTAATTTTTTGATGTAGTCTTCATGTCCGTGGGAAACGATTATCATATATACCATTTCAATGTTCTTCAGTAATAAAAtt                                      <  2:331309/81‑1 (MQ=255)         ttttGATGTAGTCTTCATGTCCGTGGGAAACGATTATCATATATACCATTTCAATGTTCTTCAGTAATAAAATTAACTAGt                               >  1:331321/1‑81 (MQ=255)            tGATGTAGTCTTCATGTCCGTGGGAAACGATTATCATATATACCATTTCAATGTTCTTCAGTAATAAAATTAACTAGTtc                             <  2:331310/80‑1 (MQ=255)              aTGTAGTCTTCATGTCCGTGGGAAACGATTATCATATATACCATTTCAATGTTCTTCAGTAATAAAATTAACTAGTtcat                           <  1:331311/80‑1 (MQ=255)              aTGTAGTCTTCATGTCCGTGGGAAACGATTATCATATATACCATTTCAATGTTCTTCAGTAATAAAATTAACTAGTtcat                           <  2:331312/80‑1 (MQ=255)                 tAGTCTTCATGTCCGTGGGAAACGATTATCATATATACCATTTCAATGTTCTTCAGTAATAAAATTAACTAGTTCATCaaa                       <  1:331313/81‑1 (MQ=255)                           gTCCGTGGGAAACGATTATCATATATACCATTTCAATGTTCTTCAGTAATAAAATTAACTAGTTCATCAAACCCAACTaa              >  2:331322/1‑80 (MQ=255)                                 gggAAACGATTATCATATATACCATTTCAATGTTCTTCAGTAATAAAATTAACTAGTTCATCAAACCCAACTAATACAttt       >  2:331327/1‑81 (MQ=255)                                    aaaCGATTATCATATATACCATTTCAATGTTCTTCAGTAATAAAATTAACTAGTTCATCAAACCCAACTAATACATTTTCa    >  1:331324/1‑81 (MQ=255)                                      aCGATTATCATATATACCATTTCAATGTTCTTCAGTAATAAAATTAACTAGTTCATCAAACCCAACTAATACATTTTCAtt  <  2:331314/81‑1 (MQ=255)                                              |                                                                         GAGTAATTTTTTGATGTAGTCTTCATGTCCGTGGGAAACGATTATTATATATACCATTTCAATGTTCTTCAGTAATAAAATTAACTAGTTCATCAAACCCAACTAATACATTTTCATT  >  NC_000913/2103333‑2103450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑