Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,107,809	C→T	R174K (AGA→AAA)	glf ←	UDP‑galactopyranose mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,107,809	0	C	T	100.0%	42.5 / NA	13	R174K (AGA→AAA)	glf	UDP‑galactopyranose mutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/8);  total (5/8)

CTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACC  >  NC_000913/2107742‑2107882                                                                    |                                                                          cTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCg                                                              <  1:331641/81‑1 (MQ=255)            cTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTtaataataa                                                   <  1:331642/81‑1 (MQ=255)                    ggAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGc                                           <  2:331643/81‑1 (MQ=255)                     gAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGc                                           >  1:331666/1‑80 (MQ=255)                          ccTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTc                                      <  2:331644/80‑1 (MQ=255)                              gATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCttt                                   <  1:331645/79‑1 (MQ=255)                              gATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTg                                 >  1:331664/1‑81 (MQ=255)                                                 atTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAGTAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCt               <  1:331646/80‑1 (MQ=255)                                                 atTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCtt              <  2:331648/81‑1 (MQ=255)                                                 atTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCtt              >  2:331670/1‑81 (MQ=255)                                                 atTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCt               <  1:331647/80‑1 (MQ=255)                                                       aTCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCg         >  1:331667/1‑80 (MQ=255)                                                             cGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAAcc  >  2:331672/1‑81 (MQ=255)                                                                    |                                                                          CTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACC  >  NC_000913/2107742‑2107882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑