| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,113,482 | T→C | K282K (AAA→AAG) | galF ← | UTP:glucose‑1‑phosphate uridylyltransferase, low activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,113,482 | 0 | T | C | 100.0% | 45.4 / NA | 14 | K282K (AAA→AAG) | galF | UTP:glucose‑1‑phosphate uridylyltransferase, low activity |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (6/8); total (6/8) | |||||||||||
ATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCA‑TTTTTTTGCCGCAGTCGTAACTGTCGCCG > NC_000913/2113405‑2113557 | aTCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCccttcctt > 2:332332/1‑80 (MQ=255) aCATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCg > 1:332325/1‑79 (MQ=255) ggTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCAt < 1:332300/81‑1 (MQ=255) gTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATa > 1:332331/1‑81 (MQ=255) aGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACt < 2:332301/80‑1 (MQ=255) ttCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTcaca > 2:332340/1‑80 (MQ=255) ttCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCt < 2:332302/80‑1 (MQ=255) gCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCa > 2:332345/1‑80 (MQ=255) cTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCata > 1:332334/1‑81 (MQ=255) tAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCata > 1:332338/1‑79 (MQ=255) ctcAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCA‑ttt < 2:332303/80‑1 (MQ=255) ccTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCA‑ttttttttgcc < 2:332304/81‑5 (MQ=255) gAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGt < 1:332305/81‑1 (MQ=255) gAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGt < 2:332306/81‑1 (MQ=255) aaCTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTa < 2:332307/81‑1 (MQ=255) ccttccttCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGcc < 1:332308/81‑1 (MQ=255) cttccttcAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCg > 1:332347/1‑81 (MQ=255) cttccttcAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCg > 2:332352/1‑81 (MQ=255) | ATCAACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCA‑TTTTTTTGCCGCAGTCGTAACTGTCGCCG > NC_000913/2113405‑2113557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |