Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	229,456	A→G	H97R (CAC→CGC)	dkgB →	methylglyoxal reductase DkgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	229,456	0	A	G	100.0%	99.6 / NA	29	H97R (CAC→CGC)	dkgB	methylglyoxal reductase DkgB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/16);  total (13/16)

AAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTG  >  NC_000913/229380‑229532                                                                             |                                                                             aaaGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTgg                                                                          >  2:49466/1‑81 (MQ=255)  aaGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCTATTATGTTGATCTGACGCTAATCCGCTGGc                                                                         >  1:49473/1‑81 (MQ=255)    gACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCg                                                                       >  1:49469/1‑81 (MQ=255)    gACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCg                                                                       <  1:49432/81‑1 (MQ=255)     aCAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCGt                                                                      <  1:49433/81‑1 (MQ=255)        aaTTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCAc                                                                   <  1:49434/81‑1 (MQ=255)          ttGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCa                                                                 <  1:49435/81‑1 (MQ=255)            gATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCaaa                                                               >  2:49476/1‑81 (MQ=255)                  aaGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCAAACGATGa                                                         <  1:49436/81‑1 (MQ=255)                         aaaGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCAAACGATGAAGtctc                                                   <  2:49438/80‑1 (MQ=255)                         aaaGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCAAACGATGAAGTctct                                                  <  1:49437/81‑1 (MQ=255)                         aaaGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCAAACGATGAAGTctct                                                  <  1:49439/81‑1 (MQ=255)                            gagagCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGtt                                               <  2:49440/81‑1 (MQ=255)                                 ccTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGaa                                            >  2:49470/1‑79 (MQ=255)                                               gTACCGATTATGTTGATCTGACGCTAATCCGCTGGCCGCCACCAAACGATGACGTCTCTGTTGAAGAGTTTATGCAGgcgc                            >  1:49467/1‑81 (MQ=255)                                                tACCGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCgct                           <  1:49441/81‑1 (MQ=255)                                                  ccGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCgctgc                         >  2:49482/1‑81 (MQ=255)                                                   cGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCgctgct                        >  1:49464/1‑81 (MQ=255)                                                   cGATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCgctgct                        >  2:49471/1‑81 (MQ=255)                                                    gATTATGTTGATCTGACGCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGctgctg                       >  1:49483/1‑81 (MQ=255)                                                              aTCTGACGCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCaaa              >  2:49472/1‑80 (MQ=255)                                                                  gACGCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAAc          >  1:49479/1‑80 (MQ=255)                                                                   aCGCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCaaaaa            <  2:49442/77‑1 (MQ=255)                                                                   aCGCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAAc          <  2:49443/79‑1 (MQ=255)                                                                   aCGCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACa         <  1:49444/80‑1 (MQ=255)                                                                     gCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAgg      <  2:49446/81‑1 (MQ=255)                                                                     gCTAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAgg      <  1:49445/81‑1 (MQ=255)                                                                       tAATCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGc    >  2:49480/1‑81 (MQ=255)                                                                         aTCCGCTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTg  <  2:49447/81‑1 (MQ=255)                                                                             |                                                                             AAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTG  >  NC_000913/229380‑229532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑