Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,141,015	T→A	T200S (ACG→TCG)	asmA ←	putative assembly protein AsmA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,141,015	0	T	A	100.0%	87.8 / NA	26	T200S (ACG→TCG)	asmA	putative assembly protein AsmA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/12);  total (14/12)

TTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTT  >  NC_000913/2140942‑2141089                                                                          |                                                                           ttAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATcg                                                                     <  1:336672/81‑1 (MQ=255)   aaTTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATcgc                                                                    <  2:336673/80‑1 (MQ=255)       tGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTg                                                                <  1:336674/80‑1 (MQ=255)         ttCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATc                                                             <  1:336675/81‑1 (MQ=255)           cAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATcgc                                                           >  2:336726/1‑81 (MQ=255)                gccgccGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATCGCGAtt                                                       >  1:336732/1‑80 (MQ=255)                   gccgTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAAcc                                                   <  1:336676/81‑1 (MQ=255)                         aaaTCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAAcccgcccg                                             >  2:336717/1‑81 (MQ=255)                          aaTCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCgg                                            <  2:336677/81‑1 (MQ=255)                               tGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAAc                                       <  2:336678/81‑1 (MQ=255)                                   gATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCa                                    >  2:336708/1‑80 (MQ=255)                                    aTAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCaaa                                  <  1:336679/81‑1 (MQ=255)                                      aaTCAGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAAt                                 >  1:336712/1‑80 (MQ=255)                                          aGAAGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAAc                             >  2:336722/1‑80 (MQ=255)                                            aaGCATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCAc                          >  2:336714/1‑81 (MQ=255)                                               cATCTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGAt                       >  1:336727/1‑81 (MQ=255)                                                  cTACCGTACCGTTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGtt                    >  1:336715/1‑81 (MQ=255)                                                   taccgtaccgTTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTg                   >  2:336728/1‑81 (MQ=255)                                                    accgtaccgtTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTgg                  >  1:336738/1‑81 (MQ=255)                                                    accgtaccgtTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTgg                  >  2:336721/1‑81 (MQ=255)                                                     ccgtaccgtTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGtt                    <  1:336680/78‑1 (MQ=255)                                                       gtaccgtTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGa               >  2:336718/1‑81 (MQ=255)                                                          ccgtTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATc             <  1:336681/80‑1 (MQ=255)                                                           cgtTAAGGGATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCtt           <  1:336682/81‑1 (MQ=255)                                                                 gggATATCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCa     >  1:336735/1‑81 (MQ=255)                                                                    atatCGAGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCAttt  <  1:336683/81‑1 (MQ=255)                                                                          |                                                                           TTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTT  >  NC_000913/2140942‑2141089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑