Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,176,866	A→G	N113N (AAT→AAC)	gatY ←	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,176,866	0	A	G	100.0%	89.0 / NA	27	N113N (AAT→AAC)	gatY	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/15);  total (12/15)

CAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCG  >  NC_000913/2176790‑2176941                                                                             |                                                                            cAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGtttt                                                                         >  2:342419/1‑81 (MQ=255) cAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGtttt                                                                         <  2:342391/81‑1 (MQ=255)            aGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGc                                                              <  1:342392/81‑1 (MQ=255)               tCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCaaa                                                           >  2:872099/1‑81 (MQ=255)                 cGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAAt                                                          <  2:342393/80‑1 (MQ=255)                   ccTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCg                                                       <  1:342394/81‑1 (MQ=255)                   ccTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCg                                                       <  1:342395/81‑1 (MQ=255)                   ccTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCg                                                       <  1:342396/81‑1 (MQ=255)                    cTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGa                                                      <  1:342397/81‑1 (MQ=255)                     tCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAg                                                     >  2:342433/1‑81 (MQ=255)                          gCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGt                                                 >  2:342444/1‑80 (MQ=255)                          gCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTc                                                <  2:342398/81‑1 (MQ=255)                             gACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAAt                                             >  1:342428/1‑81 (MQ=255)                                  cAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGa                                        >  2:342431/1‑81 (MQ=255)                                    aaGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACt                                      <  2:342400/81‑1 (MQ=255)                                    aaGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACt                                      <  2:342399/81‑1 (MQ=255)                                       cGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAg                                   >  2:342424/1‑81 (MQ=255)                                          tGGCAAAAATCCACCACCGCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAgcgc                                <  1:342401/81‑1 (MQ=255)                                               aaaaTCCACCACCTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGc                            >  1:342432/1‑80 (MQ=255)                                                          ccTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCt                <  2:342402/81‑1 (MQ=255)                                                          ccTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCt                <  2:342403/81‑1 (MQ=255)                                                          ccTCTTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCt                >  1:342429/1‑81 (MQ=255)                                                           ctctTTGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCtt               >  2:342440/1‑81 (MQ=255)                                                                tGACCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCt             <  2:342404/78‑1 (MQ=255)                                                                  aCCCGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCg        <  1:342405/81‑1 (MQ=255)                                                                   cccGTGAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGa       >  1:342439/1‑81 (MQ=255)                                                                        gAAATGTTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCg  >  1:342435/1‑81 (MQ=255)                                                                             |                                                                            CAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCG  >  NC_000913/2176790‑2176941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑