Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,191,691	C→T	A178T (GCG→ACG)	yehD ←	putative fimbrial protein YehD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,191,691	0	C	T	100.0%	54.0 / NA	17	A178T (GCG→ACG)	yehD	putative fimbrial protein YehD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/4);  total (13/4)

GGCCGCCATGGGATAGCGGCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTT  >  NC_000913/2191623‑2191765                                                                     |                                                                           ggCCGCCATGGGATAGCGGCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAAc                                                                >  2:344626/1‑81 (MQ=255)   ccgccATGGGATAGCGGCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAAc                                                                >  2:344635/1‑79 (MQ=255)    cgccATGGGATAGCGGCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCag                                                             <  1:344609/81‑1 (MQ=255)        aTGGGATAGCGGCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCagag                                                           >  1:344630/1‑79 (MQ=255)          gggATAGCGGCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCagag                                                           >  2:344629/1‑77 (MQ=255)             aTAGCGGCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATaa                                                    >  1:344640/1‑81 (MQ=255)                           tattaCCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGAtt                                      >  1:344633/1‑81 (MQ=255)                                ccTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACt                                 >  2:344631/1‑81 (MQ=255)                                       aaaCTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTAt                          >  2:344636/1‑81 (MQ=255)                                        aaCTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATc                         >  1:344641/1‑81 (MQ=255)                                                aTTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGtt                 <  2:344610/81‑1 (MQ=255)                                                          ttaATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTAttt       <  2:344611/81‑1 (MQ=255)                                                            aaTCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTg     >  2:344645/1‑81 (MQ=255)                                                             aTCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTg     <  2:344612/80‑1 (MQ=255)                                                             aTCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGc    >  1:344642/1‑81 (MQ=255)                                                                cTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCtt  >  2:344638/1‑80 (MQ=255)                                                                cTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCtt  >  2:344639/1‑80 (MQ=255)                                                                     |                                                                           GGCCGCCATGGGATAGCGGCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTT  >  NC_000913/2191623‑2191765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑