Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,197,865	C→A	intergenic (+567/‑2414)	yehF → / → yehI	putative protein YehF/DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,197,865	0	C	A	100.0%	58.7 / NA	18	intergenic (+567/‑2414)	yehF/yehI	putative protein YehF/DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/3);  total (15/3)

CGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGC  >  NC_000913/2197795‑2197937                                                                       |                                                                         cggcACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAg                                                                >  2:345502/1‑81 (MQ=255)  ggcACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAg                                                                >  1:345503/1‑80 (MQ=255)   gcACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCa                                                              <  1:345475/81‑1 (MQ=255)         aCGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTgg                                                          >  2:345490/1‑79 (MQ=255)             aaCTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGaa                                                     >  1:345506/1‑80 (MQ=255)                   ttGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCa                                              >  1:345497/1‑81 (MQ=255)                        cAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTccc                                         <  1:345476/81‑1 (MQ=255)                                  agaACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGa                               <  2:345477/81‑1 (MQ=255)                                   gaACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGa                               >  2:345508/1‑80 (MQ=255)                                   gaACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGa                               >  2:345510/1‑80 (MQ=255)                                      cAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACAcc                           >  1:345493/1‑81 (MQ=255)                                        gTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCg                         >  2:345498/1‑81 (MQ=255)                                                   aTGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTa              >  2:345499/1‑81 (MQ=255)                                                     gCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACt            >  1:345513/1‑81 (MQ=255)                                                            aCAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCa     >  2:345511/1‑81 (MQ=255)                                                               aTGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGc  >  1:345507/1‑81 (MQ=255)                                                                tGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGc  >  1:345504/1‑80 (MQ=255)                                                                tGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGc  >  2:345509/1‑80 (MQ=255)                                                                       |                                                                         CGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGC  >  NC_000913/2197795‑2197937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑