Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,209,997	T→C	L300L (TTA→CTA)	yehQ →	SWIM zinc finger domains‑containing protein YehQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,209,997	0	T	C	100.0%	171.1 / NA	48	L300L (TTA→CTA)	yehQ	SWIM zinc finger domains‑containing protein YehQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/23);  total (25/23)

GTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTCGTTG  >  NC_000913/2209919‑2210072                                                                               |                                                                            gTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTa                                                                           <  1:347417/81‑1 (MQ=255) gTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTa                                                                           <  1:347416/81‑1 (MQ=255)    gATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAAt                                                                        >  2:347454/1‑81 (MQ=255)        ccTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTgcg                                                                    <  1:347418/81‑1 (MQ=255)            ccACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGcac                                                                 <  1:347419/80‑1 (MQ=255)            ccACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGcac                                                                 <  2:347420/80‑1 (MQ=255)                 cccGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACaa                                                               >  2:347458/1‑77 (MQ=255)                  ccGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGAt                                                          <  1:347421/81‑1 (MQ=255)                      gccagccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGcc                                                      >  2:347459/1‑81 (MQ=255)                      gccagccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGcc                                                      >  1:347476/1‑81 (MQ=255)                      gccagccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGcc                                                      <  2:347422/81‑1 (MQ=255)                       ccagccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGccc                                                     <  1:347423/81‑1 (MQ=255)                        cagccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCg                                                    >  1:347470/1‑81 (MQ=255)                         agccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCgg                                                   >  2:347465/1‑81 (MQ=255)                         agccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCgg                                                   <  1:347424/81‑1 (MQ=255)                          gccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGgc                                                  <  1:347426/81‑1 (MQ=255)                          gccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGgc                                                  <  2:347427/81‑1 (MQ=255)                          gccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGgc                                                  <  2:347425/81‑1 (MQ=255)                            caCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGgcgc                                                >  1:347481/1‑81 (MQ=255)                            caCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGgcgc                                                >  1:347472/1‑81 (MQ=255)                             aCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGcgcg                                               <  1:347428/81‑1 (MQ=255)                               tataATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGa                                              >  1:347450/1‑80 (MQ=255)                                ataATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGa                                              >  1:347483/1‑79 (MQ=255)                                   aTGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTa                                         <  2:347429/81‑1 (MQ=255)                                      ccGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTg                                      >  1:347461/1‑81 (MQ=255)                                        ggAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGt                                    >  1:347453/1‑81 (MQ=255)                                          agaATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTg                                   >  2:347479/1‑80 (MQ=255)                                           gaATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTg                                   >  1:347468/1‑79 (MQ=255)                                            aaTGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAg                                >  1:347457/1‑81 (MQ=255)                                                cTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtc                             <  1:347430/80‑1 (MQ=255)                                                cTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtc                             <  2:347431/80‑1 (MQ=255)                                                 ttACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtcct                           >  2:347456/1‑81 (MQ=255)                                                    cATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTcctcct                        >  2:347460/1‑81 (MQ=255)                                                      tCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCtgt                      <  1:347432/81‑1 (MQ=255)                                                       cAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCtgtg                     >  1:347475/1‑81 (MQ=255)                                                        aGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGc                    >  1:347486/1‑81 (MQ=255)                                                        aGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGc                    <  1:347433/81‑1 (MQ=255)                                                         gCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGcc                   <  2:347434/81‑1 (MQ=255)                                                            tGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTg                >  2:347462/1‑81 (MQ=255)                                                              cGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGgc              >  2:347478/1‑81 (MQ=255)                                                               ggCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGgcg             >  2:347455/1‑81 (MQ=255)                                                               ggCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGgcg             >  1:347480/1‑81 (MQ=255)                                                                  aTTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCAc          >  2:347473/1‑81 (MQ=255)                                                                      aaCAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTc      <  1:347435/81‑1 (MQ=255)                                                                      aaCAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTc      <  1:347436/81‑1 (MQ=255)                                                                      aaCAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTc      <  1:347437/81‑1 (MQ=255)                                                                         aGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTCGtt   >  2:347469/1‑81 (MQ=255)                                                                          gTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTCGTTg  <  2:347438/81‑1 (MQ=255)                                                                               |                                                                            GTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTCGTTG  >  NC_000913/2209919‑2210072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑