Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	233,268	A→G	Y230H (TAC→CAC)	mltD ←	membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	233,268	0	A	G	100.0%	89.0 / NA	26	Y230H (TAC→CAC)	mltD	membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/12);  total (14/12)

GGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGC  >  NC_000913/233194‑233343                                                                           |                                                                            ggTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTgctgct                                                                        <  2:50156/80‑1 (MQ=255) ggTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTgctgct                                                                        <  2:50155/80‑1 (MQ=255)       tGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTc                                                                 >  2:50185/1‑81 (MQ=255)          cAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTg                                                              >  2:50194/1‑81 (MQ=255)              cGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGc                                                          <  2:50157/81‑1 (MQ=255)                  cGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAAc                                                       <  2:50158/80‑1 (MQ=255)                   gCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACgg                                                     >  2:50205/1‑81 (MQ=255)                     cATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTa                                                   <  1:50159/81‑1 (MQ=255)                      aTAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTaa                                                  >  1:50192/1‑81 (MQ=255)                       tAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAAc                                                 <  1:50160/81‑1 (MQ=255)                        aaCGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAAc                                                 >  1:50196/1‑80 (MQ=255)                           gCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGAc                                              >  1:50199/1‑80 (MQ=255)                              tGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAg                                           >  2:50204/1‑80 (MQ=255)                              tGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAg                                           >  2:50195/1‑80 (MQ=255)                              tGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAg                                           >  2:50201/1‑80 (MQ=255)                                     tttGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACTAGAAGTCCGt                                   >  1:50211/1‑81 (MQ=255)                                       tGAGAATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTg                                  <  1:50161/80‑1 (MQ=255)                                         agaATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGa                                <  2:50162/80‑1 (MQ=255)                                          gaATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGAtt                              <  2:50164/81‑1 (MQ=255)                                          gaATATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGAtt                              <  1:50163/81‑1 (MQ=255)                                           aaTATCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGAttt                             <  1:50165/81‑1 (MQ=255)                                              atCACTCAATGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTcc                           >  1:50208/1‑80 (MQ=255)                                                     aaTGCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCAcgcg                     <  1:50166/79‑1 (MQ=255)                                                        gCCAGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTg                >  2:50207/1‑81 (MQ=255)                                                           aGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTc             <  2:50168/81‑1 (MQ=255)                                                           aGCATTTTAGGCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTc             <  2:50167/81‑1 (MQ=255)                                                                     gCACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTg   >  1:50202/1‑81 (MQ=255)                                                                      cACGTGCTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGc  >  2:50203/1‑81 (MQ=255)                                                                           |                                                                            GGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTGTTCGTTTTAATTGC  >  NC_000913/233194‑233343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑