Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,221,701	T→C	M97V (ATG→GTG)	bglX ←	beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,221,701	0	T	C	100.0%	59.6 / NA	18	M97V (ATG→GTG)	bglX	beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/8);  total (10/8)

GAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTT  >  NC_000913/2221630‑2221773                                                                        |                                                                         gAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATc                                                                 >  1:349310/1‑81 (MQ=255)        gTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATg                                                          <  1:349282/81‑1 (MQ=255)                   gTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATc                                               >  1:349316/1‑81 (MQ=255)                     gcagcaCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCt                                             >  1:349324/1‑81 (MQ=255)                      cagcaCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCt                                             <  2:349283/80‑1 (MQ=255)                             tcgtAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGtt                                     <  1:349284/81‑1 (MQ=255)                                 aaGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACgg                                 >  2:349315/1‑81 (MQ=255)                                     aaagaaaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGtt                             <  2:349285/81‑1 (MQ=255)                                          aaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGaaaa                        >  1:349332/1‑81 (MQ=255)                                              agGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGc                    >  1:349320/1‑81 (MQ=255)                                                 aaTTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGcccc                 <  2:349286/81‑1 (MQ=255)                                                    tttCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAAc              >  1:349325/1‑81 (MQ=255)                                                      tCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAAcc             <  1:349287/80‑1 (MQ=255)                                                        aGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTg           <  2:349288/80‑1 (MQ=255)                                                        aGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGa          >  1:349321/1‑81 (MQ=255)                                                            ggcTTAATTCCACCACCTGATCCTGCATAGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGt      >  2:349323/1‑81 (MQ=255)                                                                 aaTTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCttt  >  2:349318/1‑80 (MQ=255)                                                                 aaTTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCtt   <  2:349289/79‑1 (MQ=255)                                                                        |                                                                         GAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTT  >  NC_000913/2221630‑2221773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑