Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,229,186	C→G	intergenic (+121/+252)	yohP → / ← dusC	putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,229,186	0	C	G	100.0%	30.4 / NA	11	intergenic (+121/+252)	yohP/dusC	putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (4/7);  total (4/7)

TCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGG  >  NC_000913/2229113‑2229253                                                                          |                                                                    tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGGCAATcgc                                                              >  2:350423/1‑81 (MQ=255)   tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGGCAATcgc                                                              <  1:350396/79‑1 (MQ=255)     aTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGGCAATcgcc                                                             <  2:350397/78‑1 (MQ=255)           ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGGCAATCGCCGCCGccccc                                                    >  2:350413/1‑81 (MQ=255)              cgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGGCAATCGCCGCCGCCCCCTGc                                                 <  1:350398/81‑1 (MQ=255)                   cTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGGCAATCGCCGCCGCCCCCTGCAcc                                              <  2:350399/79‑1 (MQ=255)                    tGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGGCAATCGCCGCCGCCCCCTGCAccccc                                           >  1:350420/1‑81 (MQ=255)                                     cATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGGCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACaa                           <  1:350400/80‑1 (MQ=255)                                         taATCACTGGCGCAAGGAGCGCGCAGGGGGCGGGCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcg                      >  2:350421/1‑81 (MQ=25)                                           aTCACTGGCGCAAGGAGCGCGCAGGGGGCGGGCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgcc                    <  2:350401/81‑1 (MQ=17)                                                             gcgcAGGGGGCGGGCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg  <  2:350403/81‑1 (MQ=14)                                                                          |                                                                    TCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGG  >  NC_000913/2229113‑2229253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑