Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,234,698	T→C	G222G (GGT→GGC)	preT →	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,234,698	0	T	C	100.0%	80.6 / NA	24	G222G (GGT→GGC)	preT	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/11);  total (13/11)

CGGTAACACACTCACCCTTGAGCAGCTGAAAGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGTTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGCCGTCGACTTCTTGCAACGTGCACGACAGGCGC  >  NC_000913/2234623‑2234771                                                                            |                                                                          cGGTAACACACTCACCCTTGAGCAGCTGAAAGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCgg                                                                      >  2:351267/1‑81 (MQ=255)       cacacTCACCCTTGAGCAGCTGAAAGCAGAAGACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTAcc                                                                <  2:351235/81‑1 (MQ=255)        acacTCACCCTTGAGCAGCTGAAAGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCg                                                               <  2:351236/81‑1 (MQ=255)                  ttGAGCAGCTGAAAGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAg                                                      >  2:351261/1‑80 (MQ=255)                   tGAGCAGCTGAAAGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCa                                                    >  1:351266/1‑81 (MQ=255)                     agcagcTGAAAGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATa                                                  <  1:351237/81‑1 (MQ=255)                        agcTGAAAGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTg                                               <  1:351238/81‑1 (MQ=255)                         gcTGAAAGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGa                                              <  1:351239/81‑1 (MQ=255)                          cTGAAAGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGAc                                             <  1:351240/81‑1 (MQ=255)                            gAAAGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGt                                           >  1:351264/1‑81 (MQ=255)                              aaGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGtt                                          >  2:351263/1‑80 (MQ=255)                              aaGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTg                                         <  1:351241/81‑1 (MQ=255)                               aGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGtt                                          >  1:351269/1‑79 (MQ=255)                                  aGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGAt                                     >  1:351268/1‑81 (MQ=255)                                     aaaCCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGc                                  >  1:351277/1‑81 (MQ=255)                                            gcgGTACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGCCGTCGAc                           >  1:351276/1‑81 (MQ=255)                                              ggTACTGGTAACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGCCGTCGActt                         <  1:351242/81‑1 (MQ=255)                                                tACTGGTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGCCGTCGActtct                       <  1:351243/81‑1 (MQ=255)                                                  ctagTCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGCCGTCGActtctt                      <  2:351244/77‑1 (MQ=255)                                                      tCACCGTGGGGTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGCCGTCGACTTCTTGCAAc                 >  1:351293/1‑81 (MQ=255)                                                              gggTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGCCGTCGACTTCTTGCAACGTGCACGa         >  1:351272/1‑81 (MQ=255)                                                               ggTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGCCGTCGACTTCTTGCAACGTGCACGa         >  2:351270/1‑80 (MQ=255)                                                               ggTTATCAAGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGCCGTCGACTTCTTGCAACGTGCACGAc        <  1:351245/81‑1 (MQ=255)                                                                       aGCGGCTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGCCGTCGACTTCTTGCAACGTGCACGACAGgcgc  >  2:351278/1‑79 (MQ=255)                                                                            |                                                                          CGGTAACACACTCACCCTTGAGCAGCTGAAAGCAGAAAACCGCGCGGTACTGGTCACCGTGGGGTTATCAAGCGGTTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGCCGTCGACTTCTTGCAACGTGCACGACAGGCGC  >  NC_000913/2234623‑2234771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑