Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,249,651	A→G	intergenic (‑33/‑66)	yeiE ← / → yeiH	putative LysR‑type transcriptional regulator YeiE/conserved inner membrane protein YeiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,249,651	0	A	G	100.0%	22.6 / NA	8	intergenic (‑33/‑66)	yeiE/yeiH	putative LysR‑type transcriptional regulator YeiE/conserved inner membrane protein YeiH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/4);  total (4/4)

CTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAA  >  NC_000913/2249582‑2249724                                                                      |                                                                          cTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAGTATATAATCaa                                                                >  2:353694/1‑81 (MQ=255)       aaaCTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAGTATATAATCAattttat                                                          <  2:353682/81‑1 (MQ=255)         aCTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAGTATATAATCAattttattt                                                        >  1:353692/1‑81 (MQ=255)                     cGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAGTATATAATCAATTTTATTTTTAAACCAGTTa                                            <  2:353683/81‑1 (MQ=255)                               tgtgCATAGTCGCTTACCACTTATAAAGATTAATTATAAGTATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCt                                  <  2:353684/81‑1 (MQ=255)                                                       aaaGATTAATTATAAGTATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGt           >  2:353693/1‑80 (MQ=255)                                                           attaattaTAAGTATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGa      >  2:353704/1‑81 (MQ=255)                                                               attaTAAGTATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGaa  <  2:353685/81‑1 (MQ=255)                                                                      |                                                                          CTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAA  >  NC_000913/2249582‑2249724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑