Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,260,779	T→C	K211R (AAA→AGA)	fruA ←	fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,260,779	0	T	C	100.0%	70.6 / NA	21	K211R (AAA→AGA)	fruA	fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/11);  total (10/11)

ATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCC  >  NC_000913/2260701‑2260850                                                                               |                                                                        aTGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTg                                                                       >  1:355402/1‑81 (MQ=255)       gAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCccgcc                                                                 <  2:355366/81‑1 (MQ=255)       gAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCccgcc                                                                 <  2:355365/81‑1 (MQ=255)         aaCGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCgg                                                               >  1:355417/1‑81 (MQ=255)            gCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGtt                                                             >  1:355400/1‑80 (MQ=255)            gCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTc                                                            <  2:355367/81‑1 (MQ=255)                        gTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGc                                                >  1:355410/1‑81 (MQ=255)                           aCGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCtt                                              <  1:355368/80‑1 (MQ=255)                               tATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCa                                         <  1:355369/81‑1 (MQ=255)                                   cgcCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAAcc                                      <  2:355370/80‑1 (MQ=255)                                      cTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCtt                                  <  1:355371/81‑1 (MQ=255)                                        gCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTTATACGGCGTTGCTTCAGCAACCGCTTTa                                >  1:355416/1‑81 (MQ=255)                                          aCTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTTCCCGCCGTTTCATACTGCGTTGCTTCAGCAACCGCTTTATc                              >  1:355409/1‑81 (MQ=255)                                          aCTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATc                              >  1:355411/1‑81 (MQ=255)                                           ctctctTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATcc                             <  2:355372/81‑1 (MQ=255)                                             ctctTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAg                           >  2:355404/1‑81 (MQ=255)                                              tctttcttACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCa                            <  1:355373/79‑1 (MQ=255)                                                             gTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTttc           <  1:355374/81‑1 (MQ=255)                                                                  cGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCCTTATCCAGTTCCTGCGCGGCTTTCTTCAg      >  2:355413/1‑81 (MQ=255)                                                                      gTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAgcgc   >  1:355419/1‑80 (MQ=255)                                                                      gTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGcc  <  1:355375/81‑1 (MQ=255)                                                                               |                                                                        ATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCC  >  NC_000913/2260701‑2260850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑