Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,263,837	T→C	intergenic (‑342/‑26)	fruB ← / → setB	fructose‑specific PTS multiphosphoryl transfer protein FruB/sugar exporter SetB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,263,837	0	T	C	100.0%	96.3 / NA	28	intergenic (‑342/‑26)	fruB/setB	fructose‑specific PTS multiphosphoryl transfer protein FruB/sugar exporter SetB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/11);  total (17/11)

AAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTC  >  NC_000913/2263760‑2263912                                                                              |                                                                            aaaTGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGcctt                                                                          <  2:355928/81‑1 (MQ=255)    tGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGccttcct                                                                       >  2:355956/1‑81 (MQ=255)    tGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGccttcct                                                                       >  1:355954/1‑81 (MQ=255)         ttttGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGccttcctcttc                                                                   >  1:355972/1‑80 (MQ=255)            tGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGt                                                               >  2:355959/1‑81 (MQ=255)            tGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGt                                                               >  1:355957/1‑81 (MQ=255)             gACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGt                                                               <  2:355929/80‑1 (MQ=255)             gACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGt                                                               >  1:355958/1‑80 (MQ=255)               cTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGAt                                                            <  1:355930/81‑1 (MQ=255)                 aTTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATc                                                           >  2:355967/1‑80 (MQ=255)                                    tCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTcc                                        <  1:355931/80‑1 (MQ=255)                                     cGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTcccc                                      >  2:355963/1‑81 (MQ=255)                                       ctgtctgtTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCg                                    >  2:355968/1‑81 (MQ=255)                                       ctgtctgtTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCg                                    <  2:355932/81‑1 (MQ=255)                                           ctgtTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGt                                >  2:355961/1‑81 (MQ=255)                                            tgtTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGtc                               >  2:355974/1‑81 (MQ=255)                                              tttATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGtctc                             <  1:355933/81‑1 (MQ=255)                                              tttATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGtc                               <  1:355934/79‑1 (MQ=255)                                                   tttCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAgcg                        >  1:355966/1‑81 (MQ=255)                                                   tttCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAgcg                        >  1:355962/1‑81 (MQ=255)                                                    ttCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAgcgc                       >  1:355964/1‑81 (MQ=255)                                                     tCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAgcgc                       <  1:355936/80‑1 (MQ=255)                                                     tCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAgcgc                       <  2:355935/80‑1 (MQ=255)                                                         aCAAGCAGCGTAAACACCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGa                     <  1:355937/78‑1 (MQ=255)                                                          cAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATc                 >  2:355970/1‑81 (MQ=255)                                                            agcagcGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGt               <  1:355938/81‑1 (MQ=255)                                                             gcagcGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGtt              >  2:355978/1‑81 (MQ=255)                                                             gcagcGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCCtt              >  2:355975/1‑81 (MQ=255)                                                                 cGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTtgac          >  1:355977/1‑81 (MQ=255)                                                                  gTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTtgacc         >  2:355973/1‑81 (MQ=255)                                                                  gTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTtgacc         >  2:355982/1‑81 (MQ=255)                                                                          cgcgCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTc  <  2:355939/80‑1 (MQ=255)                                                                              |                                                                            AAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTC  >  NC_000913/2263760‑2263912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑