Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,284,750	C→T	N125N (AAC→AAT)	yejM →	putative cardiolipin transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,284,750	0	C	T	100.0%	93.9 / NA	28	N125N (AAC→AAT)	yejM	putative cardiolipin transport protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

CTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTGAACTG  >  NC_000913/2284673‑2284825                                                                              |                                                                            cTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCa                                                                          <  2:359333/81‑1 (MQ=255)     tACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGAc                                                                       >  2:359370/1‑80 (MQ=255)       cTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGa                                                                     <  2:359335/80‑1 (MQ=255)       cTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGa                                                                     <  2:359334/80‑1 (MQ=255)          aTCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGaaaa                                                                  <  1:359336/80‑1 (MQ=255)          aTCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAAt                                                                 >  2:359372/1‑81 (MQ=255)             gATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAAt                                                                 >  2:359360/1‑78 (MQ=255)             gATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATgag                                                              <  2:359337/81‑1 (MQ=255)               tAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGAt                                                            <  1:359338/81‑1 (MQ=255)                   gAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGgcg                                                        >  1:359392/1‑81 (MQ=255)                   gAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGgcg                                                        >  1:359378/1‑81 (MQ=255)                     aGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGgcgcg                                                      <  2:359339/81‑1 (MQ=255)                             cTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGc                                              <  2:359340/81‑1 (MQ=255)                                gTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCTCGCGACTGGCAGc                                           >  2:359371/1‑81 (MQ=255)                                  ttCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCt                                          >  1:359379/1‑80 (MQ=255)                                     catctccatctTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATg                                      <  1:359341/81‑1 (MQ=255)                                        ctccatctTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGtt                                    >  2:359388/1‑80 (MQ=255)                                          ccatctTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTtcat                                 >  1:359385/1‑81 (MQ=255)                                                 aaTCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGt                           <  2:359342/80‑1 (MQ=255)                                                  aTCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTg                          <  1:359343/80‑1 (MQ=255)                                                  aTCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGc                         >  1:359384/1‑81 (MQ=255)                                                   tCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTTGCAGCTGATGTTCATCAGCGTGcc                        <  1:359344/81‑1 (MQ=255)                                                       aTCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGtt                    <  1:359345/81‑1 (MQ=255)                                                          gTCTGGCAACTGGTTATCAATCCAGACGAAAATGATATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCTGCttact                 >  2:359389/1‑79 (MQ=255)                                                          gTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGttatt                 >  2:359376/1‑81 (MQ=255)                                                                      gTTATCAATCCAGACGAAAATGAGATGGCGCACGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTGaa     <  2:359346/81‑1 (MQ=255)                                                                       ttATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTGAAc    <  1:359347/81‑1 (MQ=255)                                                                         aTCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTGAACTg  >  1:359380/1‑81 (MQ=255)                                                                              |                                                                            CTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTGAACTG  >  NC_000913/2284673‑2284825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑