Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,328,774	C→T	A342T (GCA→ACA)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,328,774	0	C	T	100.0%	102.7 / NA	29	A342T (GCA→ACA)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/12);  total (17/12)

ATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGC  >  NC_000913/2328698‑2328847                                                                             |                                                                          aTGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCt                                                                         >  2:366748/1‑79 (MQ=255)  tGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAg                                                                      >  2:366739/1‑81 (MQ=255)           aaTCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTAc                                                             >  1:366749/1‑81 (MQ=255)           aaTCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTAc                                                             >  2:366746/1‑81 (MQ=255)                ccTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTc                                                        <  2:366702/81‑1 (MQ=255)                   tCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGcc                                                     >  2:366756/1‑81 (MQ=255)                    cccGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGc                                                      <  2:366703/79‑1 (MQ=255)                    cccGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCt                                                    >  2:366740/1‑81 (MQ=255)                     ccGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCt                                                    <  2:366704/80‑1 (MQ=255)                     ccGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTc                                                   >  1:366745/1‑81 (MQ=255)                       gATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTcgc                                                 <  2:366705/81‑1 (MQ=255)                          tGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGAt                                              >  2:366742/1‑81 (MQ=255)                            gCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATc                                             <  2:366706/80‑1 (MQ=255)                             cGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCa                                           <  2:366707/81‑1 (MQ=255)                                    cTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAActct                                    <  2:366708/81‑1 (MQ=255)                                    cTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAActc                                     <  2:366709/80‑1 (MQ=255)                                     tGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTg                                   >  2:366747/1‑81 (MQ=255)                                         aaaaaGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCa                                <  2:366710/80‑1 (MQ=255)                                            aaGTGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGt                            <  1:366711/81‑1 (MQ=255)                                               tGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTAct                         >  1:366755/1‑81 (MQ=255)                                               tGCGAATCGCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTAct                         >  1:366752/1‑81 (MQ=255)                                                       gCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACg                  <  2:366712/80‑1 (MQ=255)                                                       gCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGa                 >  2:366744/1‑81 (MQ=255)                                                       gCCACGGTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGa                 >  1:366753/1‑81 (MQ=255)                                                            ggTCATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATc               >  1:366758/1‑78 (MQ=255)                                                               cATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATg         >  2:366754/1‑81 (MQ=255)                                                               cATGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATg         >  1:366750/1‑81 (MQ=255)                                                                aTGGCTTTCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATg         <  2:366713/80‑1 (MQ=255)                                                                       tCGCTGTCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGc  >  2:366765/1‑80 (MQ=255)                                                                             |                                                                          ATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGC  >  NC_000913/2328698‑2328847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑