Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,329,692	G→A	L36L (CTG→TTG)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,329,692	0	G	A	100.0%	87.6 / NA	25	L36L (CTG→TTG)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/5);  total (20/5)

AAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAAC  >  NC_000913/2329616‑2329765                                                                             |                                                                          aaaCTCCCTAACGGCGTGGTGACCGAGTCAGGTAATGCTGAGACTTTTGTCACGCCTGATGACGACAGCTGATACAATTGa                                                                       >  1:366924/1‑81 (MQ=255)    cTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCg                                                                    >  1:366909/1‑81 (MQ=255)      cccTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGtg                                                                  >  1:366905/1‑81 (MQ=255)        cTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGtgct                                                                >  2:366911/1‑81 (MQ=255)            cGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTgcg                                                             >  1:366903/1‑80 (MQ=255)               cGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCAcc                                                         >  1:366926/1‑81 (MQ=255)                   gtgAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCgcgc                                                     <  1:366878/81‑1 (MQ=255)                      aGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCgcag                                                   >  2:366920/1‑80 (MQ=255)                         gAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCAc                                                >  1:366914/1‑80 (MQ=255)                              aGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGtt                                           <  1:366879/80‑1 (MQ=255)                              aGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTg                                          >  2:366906/1‑81 (MQ=255)                                gTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGt                                         >  1:366919/1‑80 (MQ=255)                                     gCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGc                                   >  2:366922/1‑81 (MQ=255)                                        gagaCTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGtc                                >  2:366923/1‑81 (MQ=255)                                                    aCGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgt                    >  2:366930/1‑81 (MQ=255)                                                    aCGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgt                    >  2:366916/1‑81 (MQ=255)                                                      gCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgtgt                  >  1:366931/1‑81 (MQ=255)                                                        cTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACTAGTTGTAACGGCGTCTCCTCCGCAAGTGTTg                >  2:366917/1‑81 (MQ=255)                                                          gatgaCGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTgg               >  2:366937/1‑80 (MQ=255)                                                           atgaCGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTgg               <  2:366880/79‑1 (MQ=255)                                                           atgaCGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCa             <  1:366881/81‑1 (MQ=255)                                                             gacgacAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCa             >  2:366918/1‑79 (MQ=255)                                                                 acAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTaa       >  2:366925/1‑81 (MQ=255)                                                                   aGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAAc      >  2:366935/1‑80 (MQ=255)                                                                        aTACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAAc  <  2:366882/79‑1 (MQ=255)                                                                             |                                                                          AAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAAC  >  NC_000913/2329616‑2329765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑