Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,342,381	C→T	V597I (GTT→ATT)	yfaL ←	putative autotransporter adhesin YfaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,342,381	0	C	T	100.0%	86.7 / NA	25	V597I (GTT→ATT)	yfaL	putative autotransporter adhesin YfaL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

CTGCCGCATCTGAACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGC  >  NC_000913/2342306‑2342458                                                                            |                                                                              cTGCCGCATCTGAACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGc                                                                          <  1:369174/81‑1 (MQ=255)    ccGCATCTGAACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGt                                                                       <  1:369175/81‑1 (MQ=255)      gCATCTGAACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTta                                                                     <  1:369176/81‑1 (MQ=255)        aTCTGAACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTta                                                                     >  1:369202/1‑79 (MQ=255)             aaCGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTg                                                                <  2:369177/79‑1 (MQ=255)               cgcgTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTa                                                            >  2:369210/1‑81 (MQ=255)               cgcgTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTa                                                            >  2:369203/1‑81 (MQ=255)               cgcgTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTa                                                            >  1:369215/1‑81 (MQ=255)                gcgTGTCAACGTCGGATGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTAt                                                           >  2:369208/1‑81 (MQ=255)                     tCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTcc                                                       <  2:369178/80‑1 (MQ=255)                       aaCGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCaa                                                     <  1:369179/80‑1 (MQ=255)                         cGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCt                                                  <  1:369181/81‑1 (MQ=255)                         cGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCt                                                  <  2:369180/81‑1 (MQ=255)                             ggAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGc                                                >  1:369205/1‑79 (MQ=255)                                   aaTCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTc                                        >  2:369209/1‑81 (MQ=255)                                    aTCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTc                                        <  2:369182/80‑1 (MQ=255)                                        ccATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCa                                   >  1:369206/1‑81 (MQ=255)                                             aCGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATc                              <  1:369183/81‑1 (MQ=255)                                                       gttgCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTAcc                      <  1:369184/79‑1 (MQ=255)                                                       gttgCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCg                     >  2:369212/1‑80 (MQ=255)                                                              aTCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCg               <  1:369185/79‑1 (MQ=255)                                                                 ggTAAAGATAATCTGGCGGTTATAGTGGGTGTCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGAttt           <  2:369186/80‑1 (MQ=255)                                                                   tAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATttgtg        >  1:369213/1‑81 (MQ=255)                                                                     aaGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATttgtgtt      <  1:369187/81‑1 (MQ=255)                                                                         tAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGc  >  2:369214/1‑81 (MQ=255)                                                                            |                                                                              CTGCCGCATCTGAACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGC  >  NC_000913/2342306‑2342458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑